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Immunomodulatory effects of thionin Thi2.1 from Arabidopsis thaliana on bovine mammary epithelial cells
- Source :
- International Immunopharmacology. 57:47-54
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Antimicrobial peptides (AMPs) are key elements of plant defense mechanisms, resembling conserved protection strategies also present in mammals. Among the AMPs, plant thionins are particularly interesting due that display antibacterial and antifungal activities. In Arabidopsis thaliana have been described four thionins: Thi2.1, Thi2.2, Thi2.3 and Thi2.4. Work from our group shows that Thi2.1 expressed by bovine endothelial cells has direct antibacterial activity against Staphylococcus aureus mastitis isolates, bacteria able to persist inside bovine mammary epithelial cells (bMECs). Thus, the objective of this work was to analyze the immunomodulatory effects of the AMP thionin Thi2.1 from A . thaliana on bMECs during S . aureus infection. According to the results, S . aureus internalization into bMECs was reduced in cells pre-treated with Thi2.1 at 5 and 10 μg/mL during 24 h, effect related to the participation of TLR2. In addition, bMECs pre-treated with Thi2.1 (24 h) significantly increased TNF-α (~2-fold) and IL-6 (~7-fold), whereas decreased IL-10 gene expression (~0.5-fold). Interestingly, Thi2.1 inhibits the up-regulation induced by S . aureus of TNF-α and IL-10 gene expression, as well as NO production. In addition, Thi2.1 (10 μg/mL) up-regulates the expression of the chemokine IL-8 (~3-fold) in infected bMECs. Some of these effects are related to TLR2 activation. In this sense, Thi2.1 also reduces S . aureus -induced TLR2 gene expression and membrane abundance. In conclusion, Thi2.1 from A . thaliana modulates bMEC innate immune response by inducing the production of pro- and anti-inflammatory molecules while inhibits S . aureus internalization. Some of these effects are mediated by TLR2.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Staphylococcus aureus
Chemokine
media_common.quotation_subject
Immunology
Antimicrobial peptides
Arabidopsis
medicine.disease_cause
01 natural sciences
Microbiology
03 medical and health sciences
chemistry.chemical_compound
Anti-Infective Agents
Gene expression
medicine
Animals
Humans
Immunologic Factors
Immunology and Allergy
Mammary Glands, Human
Internalization
Cells, Cultured
media_common
Pharmacology
Innate immune system
biology
Arabidopsis Proteins
Tumor Necrosis Factor-alpha
Chemistry
Interleukin-8
Epithelial Cells
Staphylococcal Infections
Toll-Like Receptor 2
Interleukin-10
Thionin
TLR2
030104 developmental biology
biology.protein
Cattle
Antimicrobial Cationic Peptides
Signal Transduction
010606 plant biology & botany
Subjects
Details
- ISSN :
- 15675769
- Volume :
- 57
- Database :
- OpenAIRE
- Journal :
- International Immunopharmacology
- Accession number :
- edsair.doi.dedup.....a30e05f5070e051a08b26649423b3d04