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Chitosan modulates Pochonia chlamydosporia gene expression during nematode egg parasitism
- Source :
- RUA. Repositorio Institucional de la Universidad de Alicante, Universidad de Alicante (UA), Environmental Microbiology
- Publication Year :
- 2021
- Publisher :
- Society for Applied Microbiology, 2021.
-
Abstract
- Climate change makes plant‐parasitic nematodes (PPN) an increasing threat to commercial crops. PPN can be managed sustainably by the biocontrol fungus Pochonia chlamydosporia (Pc). Chitosan generated from chitin deacetylation enhances PPN parasitism by Pc. In this work, we investigate the molecular mechanisms of Pc for chitosan resistance and root‐knot nematode (RKN) parasitism, using transcriptomics. Chitosan and RKN modify the expression of Pc genes, mainly those involved in oxidation–reduction processes. Both agents significantly modify the expression of genes associated to 113 GO terms and 180 Pc genes. Genes encoding putative glycoproteins (Pc adhesives) to nematode eggshell, as well as genes involved in redox, carbohydrate and lipid metabolism trigger the response to chitosan. We identify genes expressed in both the parasitic and endophytic phases of the Pc lifecycle; these include proteases, chitosanases and transcription factors. Using the Pathogen—Host Interaction database (PHI‐base), our previous RNA‐seq data and RT‐PCR of Pc colonizing banana we have investigated genes expressed both in the parasitic and endophytic phases of Pc lifecycle. This work was supported by H2020 MUSA 727624 European Project.
- Subjects :
- Proteases
Nematoda
Fungus
Microbiology
Chitosan
Transcriptome
03 medical and health sciences
chemistry.chemical_compound
Chitin
Pochonia chlamydosporia
Estadística e Investigación Operativa
Gene expression
Animals
Tylenchoidea
Gene
Ecology, Evolution, Behavior and Systematics
Research Articles
030304 developmental biology
2. Zero hunger
0303 health sciences
biology
030306 microbiology
Nematode egg parasitism
Botánica
food and beverages
Plant‐parasitic nematodes
biology.organism_classification
Nematode
chemistry
Biochemistry
13. Climate action
Hypocreales
Research Article
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- RUA. Repositorio Institucional de la Universidad de Alicante, Universidad de Alicante (UA), Environmental Microbiology
- Accession number :
- edsair.doi.dedup.....a2d8ce09d9f402092466edee0d4b245e