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Integrated omics reveal novel functions and underlying mechanisms of the receptor kinase FERONIA in Arabidopsis thaliana

Authors :
Ping Wang
Natalie M Clark
Trevor M Nolan
Gaoyuan Song
Parker M Bartz
Ching-Yi Liao
Christian Montes-Serey
Ella Katz
Joanna K Polko
Joseph J Kieber
Daniel J Kliebenstein
Diane C Bassham
Justin W Walley
Yanhai Yin
Hongqing Guo
Source :
The Plant cell, vol 34, iss 7
Publication Year :
2022
Publisher :
eScholarship, University of California, 2022.

Abstract

The receptor kinase FERONIA (FER) is a versatile regulator of plant growth and development, biotic and abiotic stress responses, and reproduction. To gain new insights into the molecular interplay of these processes and to identify new FER functions, we carried out quantitative transcriptome, proteome, and phosphoproteome profiling of Arabidopsis (Arabidopsis thaliana) wild-type and fer-4 loss-of-function mutant plants. Gene ontology terms for phytohormone signaling, abiotic stress, and biotic stress were significantly enriched among differentially expressed transcripts, differentially abundant proteins, and/or misphosphorylated proteins, in agreement with the known roles for FER in these processes. Analysis of multiomics data and subsequent experimental evidence revealed previously unknown functions for FER in endoplasmic reticulum (ER) body formation and glucosinolate biosynthesis. FER functions through the transcription factor NAI1 to mediate ER body formation. FER also negatively regulates indole glucosinolate biosynthesis, partially through NAI1. Furthermore, we found that a group of abscisic acid (ABA)-induced transcription factors is hypophosphorylated in the fer-4 mutant and demonstrated that FER acts through the transcription factor ABA INSENSITIVE5 (ABI5) to negatively regulate the ABA response during cotyledon greening. Our integrated omics study, therefore, reveals novel functions for FER and provides new insights into the underlying mechanisms of FER function.

Details

Database :
OpenAIRE
Journal :
The Plant cell, vol 34, iss 7
Accession number :
edsair.doi.dedup.....a2b522c8f7a1e4dde4ab142ce08530a0