Back to Search
Start Over
Very old DNA
- Source :
- Current Opinion in Genetics & Development. 4:810-815
- Publication Year :
- 1994
- Publisher :
- Elsevier BV, 1994.
-
Abstract
- The verification of DNA sequences obtained from very old tissue sources as indeed ancient is a major point of discussion in the ancient DNA field. Proper controls and the use of the phylogenetic approach are the general methods employed for verification of the ancient DNA. Most studies have reported the recovery of extremely small amounts of nucleic acids which are sheared into rather small fragments. In addition, problems such as 'PCR jumping' can produce spurious sequence information. These observations suggest that random amplification techniques and the development of primers for highly informative but short target regions are essential for the further development of the ancient DNA field.
- Subjects :
- DNA, Bacterial
Insecta
DNA, Plant
Sequence analysis
Computational biology
Biology
Polymerase Chain Reaction
DNA sequencing
law.invention
chemistry.chemical_compound
Phylogenetics
law
Genetics
Animals
Phylogeny
Polymerase chain reaction
Phylogenetic tree
Fossils
Reproducibility of Results
DNA
Sequence Analysis, DNA
Amber
Ancient DNA
chemistry
Nucleic acid
Developmental Biology
Subjects
Details
- ISSN :
- 0959437X
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- Current Opinion in Genetics & Development
- Accession number :
- edsair.doi.dedup.....a2a55a508dc71d0d490254adf13f5e61