Back to Search
Start Over
Effects of carrier solutions on the viability and efficacy of canine adipose-derived mesenchymal stem cells
- Source :
- BMC Veterinary Research, Vol 18, Iss 1, Pp 1-14 (2022), BMC Veterinary Research
- Publication Year :
- 2022
- Publisher :
- Springer Science and Business Media LLC, 2022.
-
Abstract
- Background Mesenchymal stem cells (MSCs) have favorable characteristics that render them a potent therapeutic tool. We tested the characteristics of MSCs after temporal storage in various carrier solutions, such as 0.9% saline (saline), 5% dextrose solution (DS), heparin in saline, and Hartmann’s solution, all of which are approved by the U.S. Food and Drug Administration (FDA). Phosphate-buffered saline, which does not have FDA approval, was also used as a carrier solution. We aimed to examine the effects of these solutions on the viability and characteristics of MSCs to evaluate their suitability and efficacy for the storage of canine adipose-derived MSCs (cADMSCs). Results We stored the cADMSCs in the test carrier solutions in a time-dependent manner (1, 6, and 12 h) at 4 °C, and analyzed cell confluency, viability, proliferation, self-renewability, and chondrogenic differentiation. Cell confluency was significantly higher in 5% DS and lower in phosphate-buffered saline at 12 h compared to other solutions. cADMSCs stored in saline for 12 h showed the highest viability rate. However, at 12 h, the proliferation rate of cADMSCs was significantly higher after storage in 5% DS and significantly lower after storage in saline, compared to the other solutions. cADMSCs stored in heparin in saline showed superior chondrogenic capacities at 12 h compared to other carrier solutions. The expression levels of the stemness markers, Nanog and Sox2, as well as those of the MSC surface markers, CD90 and CD105, were also affected over time. Conclusion Our results suggest that MSCs should be stored in saline, 5% DS, heparin in saline, or Hartmann’s solution at 4 °C, all of which have FDA approval (preferable storage conditions: less than 6 h and no longer than 12 h), rather than storing them in phosphate-buffered saline to ensure high viability and efficacy.
- Subjects :
- Transplantation
Ringer's Lactate
General Veterinary
Heparin
Veterinary medicine
Organ Preservation Solutions
Proliferation
Cell Differentiation
Mesenchymal Stem Cells
Hartmann’s solution
General Medicine
5% dextrose solution
Phosphates
0.9% saline
Canine
Dogs
Adipose Tissue
Adipose-derived mesenchymal stem cells
SF600-1100
Animals
Stemness
Phosphate-buffered saline
Cells, Cultured
Cell Proliferation
Research Article
Subjects
Details
- ISSN :
- 17466148
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- BMC Veterinary Research
- Accession number :
- edsair.doi.dedup.....a28c54ed003e96551c084bcbafecbfae