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Keratin Scaffolds Containing Casomorphin Stimulate Macrophage Infiltration and Accelerate Full-Thickness Cutaneous Wound Healing in Diabetic Mice
- Source :
- Molecules, Volume 26, Issue 9, Molecules, Vol 26, Iss 2554, p 2554 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- Impaired wound healing is a major medical challenge, especially in diabetics. Over the centuries, the main goal of tissue engineering and regenerative medicine has been to invent biomaterials that accelerate the wound healing process. In this context, keratin-derived biomaterial is a promising candidate due to its biocompatibility and biodegradability. In this study, we evaluated an insoluble fraction of keratin containing casomorphin as a wound dressing in a full-thickness surgical skin wound model in mice (n = 20) with iatrogenically induced diabetes. Casomorphin, an opioid peptide with analgesic properties, was incorporated into keratin and shown to be slowly released from the dressing. An in vitro study showed that keratin-casomorphin dressing is biocompatible, non-toxic, and supports cell growth. In vivo experiments demonstrated that keratin-casomorphin dressing significantly (p &lt<br />0.05) accelerates the whole process of skin wound healing to the its final stage. Wounds covered with keratin-casomorphin dressing underwent reepithelization faster, ending up with a thicker epidermis than control wounds, as confirmed by histopathological and immunohistochemical examinations. This investigated dressing stimulated macrophages infiltration, which favors tissue remodeling and regeneration, unlike in the control wounds in which neutrophils predominated. Additionally, in dressed wounds, the number of microhemorrhages was significantly decreased (p &lt<br />0.05) as compared with control wounds. The dressing was naturally incorporated into regenerating tissue during the wound healing process. Applied keratin dressing favored reconstruction of more regular skin structure and assured better cosmetic outcome in terms of scar formation and appearance. Our results have shown that insoluble keratin wound dressing containing casomorphin supports skin wound healing in diabetic mice.
- Subjects :
- Pathology
Pharmaceutical Science
Organic chemistry
Analytical Chemistry
Mice
0302 clinical medicine
QD241-441
Tissue engineering
keratin dressing
Mice, Inbred NOD
Drug Discovery
Keratin
Medicine
Skin
chemistry.chemical_classification
0303 health sciences
integumentary system
diabetes
Tissue Scaffolds
Biomaterial
Chemistry (miscellaneous)
skin wound healing
030220 oncology & carcinogenesis
Molecular Medicine
Keratins
Casomorphin
medicine.medical_specialty
Context (language use)
tissue regeneration
casomorphin
Article
Diabetes Mellitus, Experimental
03 medical and health sciences
In vivo
Animals
Humans
Physical and Theoretical Chemistry
030304 developmental biology
Cell Proliferation
Wound Healing
Tissue Engineering
business.industry
Regeneration (biology)
Macrophages
opioids
Bandages
chemistry
Endorphins
business
Wound healing
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 26
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....a286c23a867c33736dd2858f8591582e