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Curcumin induces autophagic cell death in Spodoptera frugiperda cells
- Source :
- Pesticide Biochemistry and Physiology. 139:79-86
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- The increasing interest in the role of autophagy (type II cell death) in the regulation of insect toxicology has propelled study of investigating autophagic cell death pathways. Turmeric, the rhizome of the herb Curcuma longa (Mañjaḷ in Tamil, India and Jiānghuáng in Chinese) have been traditionally used for the pest control either alone or combination with other botanical pesticides. However, the mechanisms by which Curcuma longa or curcumin exerts cytotoxicity in pests are not well understood. In this study, we investigated the potency of Curcuma longa (curcumin) as a natural pesticide employing Sf9 insect line. Autophagy induction effect of curcumin on Spodoptera frugiperda (Sf9) cells was investigated using various techniques including cell proliferation assay, morphology analysis with inverted phase contrast microscope and Transmission Electron Microscope (TEM) analysis. Autophagy was evaluated using the fluorescent dye monodansylcadaverine (MDC). Cell death measurement was examined using 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) within the concentrations of 5-15μg/mL. Curcumin inhibited the growth of the Sf9 cells and induced autophagic cell death in a time and dose dependent manner. Staining the cells with MDC showed the presence of autophagic vacuoles while increased in a dose and time dependent manner. At the ultrastructural level transmission electron microscopy, cells revealed massive autophagy vacuole accumulation and absence of chromatin condensation. Protein expression levels of ATG8-I and ATG8-II, well-established markers of autophagy related protein were elevated in a time dependent manner after curcumin treatment. The present study proves that curcumin induces autophagic cell death in Sf9 insect cell line and this is the first report of cytotoxic effect of curcumin in insect cells and that will be utilized as natural pesticides in future.
- Subjects :
- 0301 basic medicine
Programmed cell death
Curcumin
Cell Survival
Health, Toxicology and Mutagenesis
Sf9
Vacuole
Spodoptera
Cell Line
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Microscopy, Electron, Transmission
Autophagy
Animals
Microscopy, Phase-Contrast
Curcuma
Cytotoxicity
Cell Proliferation
Cell Death
biology
Cell growth
fungi
General Medicine
biology.organism_classification
Cell biology
030104 developmental biology
chemistry
030220 oncology & carcinogenesis
Vacuoles
Insect Proteins
Agronomy and Crop Science
Subjects
Details
- ISSN :
- 00483575
- Volume :
- 139
- Database :
- OpenAIRE
- Journal :
- Pesticide Biochemistry and Physiology
- Accession number :
- edsair.doi.dedup.....a25c7bdad5806be35663d626391add9e