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Adipocyte-Derived Plasma Protein, Adiponectin, Suppresses Lipid Accumulation and Class A Scavenger Receptor Expression in Human Monocyte-Derived Macrophages
- Source :
- Circulation. 103:1057-1063
- Publication Year :
- 2001
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2001.
-
Abstract
- Background —Excessive lipid accumulation in macrophages plays an important role in the development of atherosclerosis. Recently, we discovered an adipocyte-specific plasma protein, adiponectin, that is decreased in patients with coronary artery disease. We previously demonstrated that adiponectin acts as a modulator for proinflammatory stimuli and inhibits monocyte adhesion to endothelial cells. The present study investigated the effects of adiponectin on lipid accumulation in human monocyte-derived macrophages. Methods and Results —Human monocytes were differentiated into macrophages by incubation in human type AB serum for 7 days, and the effects of adiponectin were investigated at different time intervals. Treatment with physiological concentrations of adiponectin reduced intracellular cholesteryl ester content, as determined using the enzymatic, fluorometric method. The adiponectin-treated macrophages contained fewer lipid droplets stained by oil red O. Adiponectin suppressed the expression of the class A macrophage scavenger receptor (MSR) at both mRNA and protein levels by Northern and immunoblot analyses, respectively, without affecting the expression of CD36, which was quantified by flow cytometry. Adiponectin reduced the class A MSR promoter activity, as measured by luciferase reporter assay. Adiponectin treatment dose-dependently decreased class A MSR ligand binding and uptake activities. The mRNA level of lipoprotein lipase as a marker of macrophage differentiation was decreased by adiponectin treatment, but that of apolipoprotein E was not altered. Adiponectin was detected around macrophages in the human injured aorta by immunohistochemistry. Conclusions —The adipocyte-derived plasma protein adiponectin suppressed macrophage-to-foam cell transformation, suggesting that adiponectin may act as a modulator for macrophage-to-foam cell transformation.
- Subjects :
- CD36 Antigens
medicine.medical_specialty
Biology
Monocytes
Proinflammatory cytokine
chemistry.chemical_compound
Apolipoproteins E
Physiology (medical)
Internal medicine
Adipocyte
Lipid droplet
Adipocytes
medicine
Humans
Macrophage
RNA, Messenger
Receptors, Immunologic
Scavenger receptor
Receptor
Cells, Cultured
Receptors, Scavenger
Adiponectin
Macrophages
Proteins
Scavenger Receptors, Class A
Cell Differentiation
Blood Proteins
Lipid Metabolism
Peptide Fragments
Lipoproteins, LDL
Lipoprotein Lipase
Endocrinology
chemistry
Cholesteryl ester
Intercellular Signaling Peptides and Proteins
Cholesterol Esters
Cardiology and Cardiovascular Medicine
hormones, hormone substitutes, and hormone antagonists
Foam Cells
Subjects
Details
- ISSN :
- 15244539 and 00097322
- Volume :
- 103
- Database :
- OpenAIRE
- Journal :
- Circulation
- Accession number :
- edsair.doi.dedup.....a2573c4202cb9d15e4446f57b975ab00