Back to Search
Start Over
A new fluorescent probe for neutral to alkaline pH and imaging application in live cells
- Source :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 261
- Publication Year :
- 2021
-
Abstract
- A new pH-sensitive fluorescent probe NAP-MDA was designed and synthesized. NAP-MDA consists of 1,8-naphthalimide as fluorophore, morpholine and N,N-dimethylethylenediamine as pH-responsive groups. Due to the photoinduced electron transfer (PET) mechanism, the fluorescence of 1, 8-naphthalimide was thoroughly quenched under alkaline condition (pH > 10.0), however, NAP-MDA displayed increasing fluorescence as the rise of acidity. Notably, NAP-MDA possessed an excellent linear dependence with neutral to alkaline pH (7.2–9.4), with a pKa of 8.38. NAP-MDA had good photostability and reversibility. Meanwhile, the probe was selective to pH without interference from common reactive species, temperature and viscosity. Fluorescent testing strips were fabricated with NAP-MDA and were successfully utilized to visualize the different pH with a handhold UV lamp. Confocal fluorescence imaging in live cells demonstrated that NAP-MDA mainly fluoresced in lysosomes, and could be applied for quantification of the pH within live cells.
- Subjects :
- Fluorescence-lifetime imaging microscopy
Fluorophore
Confocal
02 engineering and technology
010402 general chemistry
Photochemistry
01 natural sciences
Photoinduced electron transfer
Fluorescence
Analytical Chemistry
chemistry.chemical_compound
Morpholine
mental disorders
Instrumentation
Spectroscopy
Fluorescent Dyes
Chemistry
musculoskeletal, neural, and ocular physiology
fungi
Optical Imaging
Hydrogen-Ion Concentration
021001 nanoscience & nanotechnology
Atomic and Molecular Physics, and Optics
0104 chemical sciences
Naphthalimides
0210 nano-technology
Lysosomes
psychological phenomena and processes
Subjects
Details
- ISSN :
- 18733557
- Volume :
- 261
- Database :
- OpenAIRE
- Journal :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
- Accession number :
- edsair.doi.dedup.....a21102db210e0b1fffe71f7a88e17a7a