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Postsynaptic Element Contributes to the Delay in Synaptogenesis in Synapsin I-Deficient Neurons
- Source :
- Molecular and Cellular Neuroscience. 8:286-299
- Publication Year :
- 1996
- Publisher :
- Elsevier BV, 1996.
-
Abstract
- In a previous study, we reported a retardation in process outgrowth and synapse formation in cultured hippocampal neurons from synapsin I-deficient mice. Here we investigated whether this delay in synaptogenesis was attributable to pre- or postsynaptic elements. The experimental paradigm used in this study involved the establishment of heterochronic cocultures of neurons from wild-type and synapsin I-deficient mice. Newly cultured axons from wild-type and synapsin I-deficient neurons established synapses with mature wild-type postsynaptic elements after 24 and 72 h, respectively. In contrast, synapsin I-deficient postsynaptic elements were able to receive synapses only after 9 days in culture, representing a 5-day delay compared to controls. The results suggest a broad role for synapsin I in the structural development of the synapse, participating directly or indirectly in the maturation of both presynaptic and postsynaptic sites.
- Subjects :
- Neurons
Synapsin I
Time Factors
Synaptogenesis
Cell Count
Dendrites
Cell Biology
Synapsin
Hippocampal formation
Biology
Synapsins
Inhibitory postsynaptic potential
Hippocampus
Axons
Synapse
Mice
Cellular and Molecular Neuroscience
nervous system
Postsynaptic potential
Synapses
Animals
Molecular Biology
Postsynaptic density
Neuroscience
Cells, Cultured
Subjects
Details
- ISSN :
- 10447431
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Molecular and Cellular Neuroscience
- Accession number :
- edsair.doi.dedup.....a1ec02a007f45b24662d43f796c64d4c