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PFA: Program for the Quantitative Assessment of Cell Metabolism by Spectral Data Analysis
- Source :
- Bioinformation
- Publication Year :
- 2008
- Publisher :
- Biomedical Informatics, 2008.
-
Abstract
- Assessment of mitochondrial oxidative metabolism has wide-ranging importance, from pharmacokinetic analysis to studies in cell viability and apoptosis. Here we present the Perfusion File Analyzer (PFA) application for the real-time analysis of spectral data to measure cytochrome c reduction, cytochrome a3 reduction, and other parameters important to cellular metabolism, which are collected during tissue perfusion experiments. Our current efforts are focused on quantitating changes in mitochondrial function by normalizing baseline drift of spectral data while addressing two major challenges: (1) a lack of real-time feedback from the system when aiming is compromised, and (2) an inability to adjust calculated data in the event of spectral shift. PFA has been developed to address these issues, and is currently used for quality assessment of human islets prior to clinical transplantation.
- Subjects :
- Oxidative metabolism
biology
business.industry
Cytochrome c
data analysis
pharmacokinetics analysis
General Medicine
cell
Spectral shift
Bioinformatics
Pharmacokinetic analysis
Transplantation
Cell metabolism
oxidative metabolism
Quantitative assessment
biology.protein
Medicine
business
Spectral data
Software
Subjects
Details
- ISSN :
- 09732063 and 09738894
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- Bioinformation
- Accession number :
- edsair.doi.dedup.....a1e3066338f733a39ca4f6579c54df05