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LncRNA PCAT-1 upregulates RAP1A through modulating miR-324-5p and promotes survival in lung cancer
- Source :
- Archives of Medical Science : AMS
- Publication Year :
- 2018
-
Abstract
- Introduction Lung cancer is the malignant tumor with the fastest increase in morbidity and mortality and the greatest threat to human health and life. Long non-coding RNA (lncRNA) is emerging as an important regulator in many cancers. Recently, it was found that lncRNA prostate cancer associated transcript 1 (PCAT-1) was up-regulated in lung cancer, playing oncogenic roles. However, the underlying regulatory mechanism of PCAT-1 remains unknown. Material and methods The expression levels of PCAT-1 and miR-324-5p were analyzed by real-time PCR, and RAP1A expression was determined by western blotting. RNA pull-down, luciferase and western blotting assays were used to examine the target relationship between PCAT-1 and miR-324-5p or that between miR-324-5p and RAP1A. The functional effects of PCAT-1 and miR-324-5p were examined using cell viability and cell apoptosis assays. Results PCAT-1 overexpression remarkably promoted cell proliferation and suppressed cell apoptosis. Mechanistic investigations demonstrated that PCAT-1 can interact with miR-324-5p and repress its expression, thereby increasing the expression of its target RAP1A. Additionally, rescue experiments revealed that PCAT-1 served as an oncogene partly through sponging miR-324-5p and upregulating RAP1A in lung cancer cells. Conclusions Our findings demonstrate that on account of the dual function of pro-proliferation and anti-apoptosis, PCAT-1/miR-324-5p/RAP1A may be novel candidates for application in the diagnosis, prognosis and therapy of lung cancer.
- Subjects :
- Oncogene
Cell growth
business.industry
RNA
General Medicine
medicine.disease
lncRNA PCAT-1
cell survival
Prostate Cancer Associated Transcript 1
Blot
03 medical and health sciences
lung cancer
0302 clinical medicine
Basic Research
Apoptosis
Cancer research
medicine
miR-324-5p
RAP1A
030212 general & internal medicine
Viability assay
Lung cancer
business
Subjects
Details
- ISSN :
- 17341922
- Volume :
- 16
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Archives of medical science : AMS
- Accession number :
- edsair.doi.dedup.....a1e13f10f0019726c6abf90c8d8ccc0b