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Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules
- Source :
- Scopus, Repositório Institucional da UNESP, Universidade Estadual Paulista (UNESP), instacron:UNESP
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Made available in DSpace on 2021-06-25T10:24:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-03-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Rhipicephalus sanguineus sensu lato (s.l.), popularly known as ‘brown dog tick’, is the primary vector of pathogens affecting dogs worldwide. To enter the host’s organism, these pathogens utilise the anticoagulant, antiplatelet, anti-inflammatory and immunomodulatory actions of compounds present in the tick’s saliva; such compounds are released by the ectoparasite in order to attach and feed on dogs. Nitric oxide (NO) is one of the regulatory factors in inflammation, apoptosis and immunomodulation. Here, we evaluated the in vitro activity of salivary gland extract of female dog ticks on the macrophage-derived J774 cell line, with and without lipopolysaccharide (LPS) stimulation. Cultures were evaluated for possible morphological alterations caused by exposure to the extract. There was no apparent in vitro cytotoxicity of the extract. Also, the NO secretory response in the non-LPS-stimulated cells was not inhibited. On the other hand, the extract presented modulatory action in the cultures of LPS-stimulated cells at a concentration of 0.1 μg/mL, possibly through macrophage activation, and induced a significant decrease in NO secretion. These results confirm the modulatory potential of bioactive molecules in the salivary glands of R. sanguineus ticks. Department of Biology Institute of Biosciences São Paulo State University – UNESP Department of Genetics Evolution and Bioagents Institute of Biology University of Campinas – UNICAMP Department of Biology Institute of Biosciences São Paulo State University – UNESP FAPESP: 2014/02843-8 FAPESP: 2015/20745-6 FAPESP: 2018/2999-9 FAPESP: 2019/02831-3 CNPq: 300625/2012-0
- Subjects :
- 0106 biological sciences
Saliva
Lipopolysaccharide
Rhipicephalus sanguineus
Biology
01 natural sciences
Salivary Glands
030308 mycology & parasitology
Microbiology
Immunomodulation
03 medical and health sciences
chemistry.chemical_compound
Ticks
Dogs
Ectoparasite-host relationships
parasitic diseases
medicine
Animals
Macrophage
Secretion
Dog Diseases
0303 health sciences
Ecology
Salivary gland
Plant Extracts
Nitric oxide
General Medicine
biology.organism_classification
In vitro
010602 entomology
medicine.anatomical_structure
chemistry
Animal ecology
Insect Science
Female
Subjects
Details
- ISSN :
- 15729702 and 01688162
- Volume :
- 83
- Database :
- OpenAIRE
- Journal :
- Experimental and Applied Acarology
- Accession number :
- edsair.doi.dedup.....a1c9dc72fae24d9fa308db360dd827c6