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Effect of amniotic fluid stem cell transplantation on the recovery of bladder dysfunction in spinal cord-injured rats
- Source :
- Scientific Reports, Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- The effects of human amniotic fluid stem cell (hAFSC) transplantation on bladder function and molecular changes in spinal cord-injured (SCI) rats were investigated. Four groups were studied: sham and SCI plus phosphate-buffered saline (SCI + PBS), human embryonic kidney 293 (HEK293) cells, and hAFSCs transplantation. In SCI + PBS rat bladders, cystometry showed increased peak voiding pressure, voiding volume, bladder capacity, residual volume, and number of non-voiding contractions, and the total elastin/collagen amount was increased but collagen concentration was decreased at days 7 and 28. Immunoreactivity and mRNA levels of IGF-1, TGF-β1, and β3-adrenoceptor were increased at days 7 and/or 28. M2 immunoreactivity and M3 mRNA levels of muscarinic receptor were increased at day 7. M2 immunoreactivity was increased, but M2/M3 mRNA and M3 immunoreactivity levels were decreased at day 28. Brain derived-neurotrophic factor mRNA was increased, but immunoreactivity was decreased at day 7. HEK293 cell transplantation caused no difference compared to SCI + PBS group. hAFSCs co-localized with neural cell markers and expressed BDNF, TGF-β1, GFAP, and IL-6. The present results showed that SCI bladders released IGF-1 and TGF-β1 to stimulate elastin and collagen for bladder wall remodelling, and hAFSC transplantation improved these changes, which involved the mechanisms of BDNF, muscarinic receptors, and β3-adrenoceptor expression.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Amniotic fluid
Bladder
Urinary Bladder
lcsh:Medicine
Spinal cord diseases
Real-Time Polymerase Chain Reaction
Article
Rats, Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Internal medicine
Muscarinic acetylcholine receptor
Animals
Humans
Medicine
lcsh:Science
Spinal Cord Injuries
Kidney
Microscopy, Confocal
Multidisciplinary
biology
medicine.diagnostic_test
business.industry
lcsh:R
Urinary Bladder Diseases
Cystometry
Amniotic Fluid
Spinal cord
Elastin
Rats
Transplantation
HEK293 Cells
030104 developmental biology
medicine.anatomical_structure
Endocrinology
biology.protein
lcsh:Q
Female
Collagen
Stem cell
business
030217 neurology & neurosurgery
Stem Cell Transplantation
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....a1bdd3ffdb1a4d91c6b5528d971317c7