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Monoclonal antibodies recognizing lipid-laden cells and extracellular regions with lipid-deposits in atherosclerotic aorta
- Source :
- Virchows Archiv. A, Pathological anatomy and histopathology. 410(2)
- Publication Year :
- 1986
-
Abstract
- Monoclonal antibodies against lipid-laden cells and against extracellular regions of lipid-deposits in atherosclerotic aorta were prepared. Mice were immunized with a delipidated homogenate of atherosclerotic aorta of Watanabe-heritable hyperlipidemic rabbits. Hybridomas were obtained by fusion and cultured in hypoxanthine, aminopterin and thymidine selection medium. Specific antibodies were selected by indirect immunohistochemical staining of frozen sections of atherosclerotic aorta. Nine clones that produced antibodies that stained the atherosclerotic intima exclusively were selected and cloned by limiting dilution. Finally two clones (FCR1a/201F, FCR1b/ 904B) producing antibodies specific to lipid-laden cells and one clone (EMR1a/212D) producing an antibody specific to regions with lipid deposits in the extracellular matrix were established. These monoclonal antibodies may help in understanding how lipids accumulate in atherosclerosis.
- Subjects :
- Pathology
medicine.medical_specialty
medicine.drug_class
Arteriosclerosis
Aminopterin
Monoclonal antibody
Pathology and Forensic Medicine
Extracellular matrix
medicine
Extracellular
Animals
Molecular Biology
Foam cell
Hybridomas
biology
Macrophages
Antibodies, Monoclonal
Cell Biology
General Medicine
Lipid Metabolism
Lipids
Extracellular Matrix
biology.protein
Immunohistochemistry
Female
Rabbits
Antibody
Clone (B-cell biology)
medicine.drug
Foam Cells
Subjects
Details
- ISSN :
- 01747398
- Volume :
- 410
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Virchows Archiv. A, Pathological anatomy and histopathology
- Accession number :
- edsair.doi.dedup.....a1b09017a79064d4c4e01708c8aa006d