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Conformational state of human cardiac 5-HT4(g) receptors influences the functional effects of polyclonal anti-5-HT4 receptor antibodies
- Source :
- Biochemical Pharmacology, Biochemical Pharmacology, Elsevier, 2007, 73, pp 964-971, Biochemical Pharmacology, Elsevier, 2007, 73 (7), pp.964-971. ⟨10.1016/j.bcp.2006.12.009⟩
- Publication Year :
- 2007
- Publisher :
- HAL CCSD, 2007.
-
Abstract
- International audience; The functional effects of the anti-G21V antibody directed against the second extracellular loop of human heart S-HT4 receptors can differ when the receptors are expressed in different cell lines. Here, we extend these studies to show variation in the responses of 5-HT4(g) receptors to the antibody within the same expression system. In a previous report no effect of the anti-G21V antibodies had been shown upon 5-HT4(g) receptors expressed in CHO cells. Here the same antibodies alone or when added before 5-HT had a functional "inverse-agonist like" effect upon 5-HT4(g) receptors expressed in a separate line of CHO cells. Although these CHO cells showed a lower efficacy of cAMP production evoked by 5-HT than the previous report they express a similar hS-HT4(g) receptor density. Inhibition of either phosphodiesterases or Gi proteins had no effect upon the action of the antibody. Conformational states of the 5-HT4 receptor and/or equilibrium between different states of receptors may then determine the functional effect of antibodies against this receptor. (c) 2006 Elsevier Inc. All rights reserved.
- Subjects :
- Protein Conformation
conformational state
Immune receptor
Biology
Biochemistry
Antibodies
03 medical and health sciences
Cricetulus
Antibody Specificity
Cricetinae
antibody
Animals
Humans
Protease-activated receptor
5-HT4 receptor
Receptor
5-HT receptor
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
G protein-coupled receptor
Pharmacology
0303 health sciences
G protein-coupled receptor kinase
[ SDV ] Life Sciences [q-bio]
Myocardium
030302 biochemistry & molecular biology
CHO cells
[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
musculoskeletal system
Molecular biology
inverse-agonist
serotonin
Metabotropic receptor
5-HT1 receptor
Receptors, Serotonin, 5-HT4
Subjects
Details
- Language :
- English
- ISSN :
- 00062952 and 18732968
- Database :
- OpenAIRE
- Journal :
- Biochemical Pharmacology, Biochemical Pharmacology, Elsevier, 2007, 73, pp 964-971, Biochemical Pharmacology, Elsevier, 2007, 73 (7), pp.964-971. ⟨10.1016/j.bcp.2006.12.009⟩
- Accession number :
- edsair.doi.dedup.....a19eccba777531ddba2c2d197c1d0782