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Serum Mast Cell Tryptase as a Marker of Posttraumatic Joint Contracture in a Rabbit Model
- Source :
- Journal of Orthopaedic Trauma. 31:e86-e89
- Publication Year :
- 2017
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2017.
-
Abstract
- OBJECTIVES Mast cells have been identified as key mediators of posttraumatic joint contracture, and stabilizing medications (ketotifen) have been shown to decrease contracture severity. Serum mast cell tryptase (SMCT) levels are used clinically to monitor mast cell-mediated conditions. The goals of this study were to determine if SMCT levels are elevated in the setting of joint contracture, if they can be decreased in association with ketotifen therapy, and if they correlate with contracture severity. METHODS This study used a previously developed rabbit model in which 39 animals were divided into 4 groups: operatively created joint contracture (ORC, n = 13), operatively created contracture treated with ketotifen at 2 doses (KF0.5, n = 9; KF1.0, n = 9), and healthy rabbits (NC, n = 8). Range of motion measures were performed at 8 weeks after the surgery. Serum samples were collected on postoperative days 1, 3, 5, 7, 21, 35, and 49. SMCT levels were measured using a rabbit-specific enzyme-linked immunosorbent assay. RESULTS Levels of SMCT were highest in the operatively created joint contracture group and were significantly greater compared with both ketotifen groups (P < 0.001). Levels were highest at postoperative day 1 with a trend to decrease over time. A positive correlation between SMCT levels and contracture severity was observed in all operative groups (P < 0.05). CONCLUSIONS Levels of SMCT are elevated in the setting of joint contracture, decreased in association with ketotifen therapy, and positively correlated with contracture severity. This is the first study to establish a relationship between SMCT and joint injury. Measurement of SMCT may be valuable in identifying those at risk of posttraumatic joint contracture.
- Subjects :
- 0301 basic medicine
Ketotifen
medicine.medical_specialty
Contracture
Knee Injuries
Positive correlation
Sensitivity and Specificity
Gastroenterology
Mast cell tryptase
03 medical and health sciences
0302 clinical medicine
Internal medicine
Animals
Medicine
Orthopedics and Sports Medicine
Joint Contracture
030222 orthopedics
business.industry
Reproducibility of Results
General Medicine
Serum samples
030104 developmental biology
Anesthesia
Rabbit model
Tryptases
Surgery
Rabbits
medicine.symptom
business
Range of motion
Biomarkers
medicine.drug
Subjects
Details
- ISSN :
- 08905339
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Journal of Orthopaedic Trauma
- Accession number :
- edsair.doi.dedup.....a19c0fde22ebd37667d8002d18de46a7