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Anti-rheumatoid arthritic activity of flavonoids from Daphne genkwa
- Source :
- Phytomedicine. 21:830-837
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- The aim of the study was to investigate the anti-rheumatoid arthritic activity of four flavonoids from Daphne genkwa (FFD) in vivo and in vitro. Flavonoids of D. genkwa were extracted by refluxing with ethanol and purified by polyamide resin. An in vivo carrageenan-induced paw edema model, tampon-granuloma model and Freund's complete adjuvant (FCA)-induced arthritis mouse model were used to evaluate the anti-rheumatoid arthritic activities of FFD. Moreover, nitric oxide (NO) release and neutral red uptake (NRU) in lipopolysaccharide (LPS)-induced murine macrophage RAW264.7 cells were used to evaluate the anti-inflammatory effect in vitro. In addition, antioxidant effect of FFD was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. A high dose of FFD significantly reduced the degree of acute inflammatory paw edema in mice as a response to carrageenan administration (p
- Subjects :
- Lipopolysaccharides
Male
Daphne genkwa
Antioxidant
Lipopolysaccharide
DPPH
medicine.medical_treatment
Freund's Adjuvant
Anti-Inflammatory Agents
Pharmaceutical Science
Arthritis
Pharmacology
Carrageenan
Nitric Oxide
Antioxidants
Nitric oxide
Arthritis, Rheumatoid
Mice
chemistry.chemical_compound
Picrates
In vivo
Drug Discovery
Animals
Edema
Medicine
Flavonoids
Inflammation
Dose-Response Relationship, Drug
biology
Plant Extracts
business.industry
Macrophages
Biphenyl Compounds
biology.organism_classification
medicine.disease
Arthritis, Experimental
Rats
Complementary and alternative medicine
chemistry
Antirheumatic Agents
Immunology
Molecular Medicine
Daphne
Female
business
Phytotherapy
Subjects
Details
- ISSN :
- 09447113
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Phytomedicine
- Accession number :
- edsair.doi.dedup.....a18bfe038e372aee39a3ef948cfaf83e