Back to Search
Start Over
Involvement of NEAT1/miR‐133a axis in promoting cervical cancer progression via targeting SOX4
- Source :
- Journal of Cellular Physiology. 234:18985-18993
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- NEAT1 is an important tumor oncogenic gene in various tumors. Nevertheless, its involvement remains poorly studied in cervical cancer. Our study explored the functional mechanism of NEAT1 in cervical cancer. NEAT1 level in several cervical cancer cells was quantified and we found NEAT1 was greatly upregulated in vitro. NEAT1 knockdown inhibited cervical cancer development through repressing cell proliferation, colony formation, capacity of migration, and invasion and also inducing the apoptosis. For another, microRNA (miR)-133a was downregulated in cervical cancer cells and NEAT1 negatively modulated miR-133a expression. Subsequently, we validated that miR-133a functioned as a potential target of NEAT1. Meanwhile, SOX4 is abnormally expressed in various cancers. SOX4 was able to act as a downstream target of miR-133a and silencing of SOX4 can restrain cervical cancer progression. In addition, in vivo assays were conducted to prove the role of NEAT1/miR-133a/SOX4 axis in cervical cancer. These findings implied that NEAT1 served as a competing endogenous RNA to sponge miR-133a and regulate SOX4 in cervical cancer pathogenesis. To sum up, it was implied that NEAT1/miR-133a/SOX4 axis was involved in cervical cancer development.
- Subjects :
- 0301 basic medicine
Carcinogenesis
Physiology
Clinical Biochemistry
Uterine Cervical Neoplasms
SOXC Transcription Factors
03 medical and health sciences
SOX4
0302 clinical medicine
Downregulation and upregulation
Cell Movement
Cell Line, Tumor
microRNA
medicine
Humans
Gene silencing
Cell Proliferation
Cervical cancer
Gene knockdown
Cell growth
business.industry
Competing endogenous RNA
Cell Biology
medicine.disease
Gene Expression Regulation, Neoplastic
MicroRNAs
030104 developmental biology
030220 oncology & carcinogenesis
Disease Progression
Cancer research
Female
RNA, Long Noncoding
business
HeLa Cells
Subjects
Details
- ISSN :
- 10974652 and 00219541
- Volume :
- 234
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Physiology
- Accession number :
- edsair.doi.dedup.....a1897f2e79f5d8525c4a222828d83fb0