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Genomic organization and allelic polymorphism of the human killer cell inhibitory receptor gene KIR 103
- Source :
- Tissue Antigens. 49:564-573
- Publication Year :
- 1997
- Publisher :
- Wiley, 1997.
-
Abstract
- Killer cell inhibitory receptors (KIR) belong to the immunoglobulin superfamily of molecules and are expressed on natural killer (NK) cells. The KIRs of the p58/p50 family have two immunoglobulin domains and are ligands for HLA-Cw antigens, whereas the p70/p70 delta family has three immunoglobulin domains and comprises ligands for HLA-B antigens and possibly some HLA-A antigens. Members of a third KIR family, KIR103, have two immunoglobulin domains but have highest nucleotide sequence homology to the p70 family. The ligands for KIR103 on target cells are currently unknown. We here report the complete genomic organization of KIR103. It spans about 12 kb of DNA and consists of eight exons of which exon 1 and exon 2 encode the leader sequence. Exon 3 encodes the first immunoglobulin domain (gamma 1), and exon 4 encodes the main part of the second immunoglobulin domain (gamma 3), which also contains sequences contributed by exon 5 and exon 6. Exon 6 encodes the transmembrane domain, whereas exons 7 and 8 encode most of the cytoplasmic domain. KIR103 is polymorphic, and two alleles, 103AS and 103LP, are defined in this study. Additional full-length cDNA clones for KIR103 have been isolated and are shown to be formed by alternative mRNA splicing with exon skipping. Some of these truncated KIR103 cDNA could encode shorter transmembrane molecules, whereas others lack the transmembrane domain and are candidate genes for secreted KIR products. KIR103 is localized to the KIR genetic region on chromosome 19q13.4.
- Subjects :
- DNA, Complementary
Molecular Sequence Data
Immunology
Immunoglobulin domain
Biology
Biochemistry
Cell Line
Exon
Receptors, KIR
Complementary DNA
Genetics
Humans
Immunology and Allergy
Amino Acid Sequence
Receptors, Immunologic
Gene
Alleles
Genomic organization
Genome
Polymorphism, Genetic
Base Sequence
Chromosome Mapping
General Medicine
Molecular biology
Exon skipping
Killer Cells, Natural
Alternative Splicing
Transmembrane domain
Receptors, KIR2DL3
Immunoglobulin superfamily
Chromosomes, Human, Pair 19
Subjects
Details
- ISSN :
- 13990039 and 00012815
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- Tissue Antigens
- Accession number :
- edsair.doi.dedup.....a17de33e2f2cfbb0a4a70d06c8c2228d