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Neuronal loss, demyelination and volume change in the multiple sclerosis neocortex
- Source :
- Carassiti, D, Altmann, D R, Petrova, N, Pakkenberg, B, Scaravilli, F & Schmierer, K 2018, ' Neuronal loss, demyelination and volume change in the multiple sclerosis neocortex ', Neuropathology and Applied Neurobiology, vol. 44, no. 4, pp. 377-390 . https://doi.org/10.1111/nan.12405
- Publication Year :
- 2017
- Publisher :
- Wiley, 2017.
-
Abstract
- AIMS: Indices of brain volume [grey matter, white matter (WM), lesions] are being used as outcomes in clinical trials of patients with multiple sclerosis (MS). We investigated the relationship between cortical volume, the number of neocortical neurons estimated using stereology and demyelination.METHODS: Nine MS and seven control hemispheres were dissected into coronal slices. On sections stained for Giemsa, the cortex was outlined and optical disectors applied using systematic uniform random sampling. Neurons were counted using an oil immersion objective (× 60) following stereological principles. Grey and WM demyelination was outlined on myelin basic protein immunostained sections, and expressed as percentages of cortex and WM respectively.RESULTS: In MS, the mean number of neurons was 14.9 ± 1.9 billion vs. 24.4 ± 2.4 billion in controls (P < 0.011), a 39% difference. The density of neurons was smaller by 28% (P < 0.001) and cortical volume by 26% (P = 0.1). Strong association was detected between number of neurons and cortical volume (P < 0.0001). Demyelination affected 40 ± 13% of the MS neocortex and 9 ± 12% of the WM, however, neither correlated with neuronal loss. Only weak association was detected between number of neurons and WM volume.CONCLUSION: Neocortical neuronal loss in MS is massive and strongly predicted by cortical volume. Cortical volume decline detected in vivo may be similarly indicative of neuronal loss. Lack of association between neuronal density and demyelination suggests these features are partially independent, at least in chronic MS.
- Subjects :
- Male
0301 basic medicine
Pathology
medicine.medical_specialty
Multiple Sclerosis
Histology
Cell Count
Neocortex
Stereology
Grey matter
Pathology and Forensic Medicine
White matter
03 medical and health sciences
0302 clinical medicine
Physiology (medical)
Cortex (anatomy)
medicine
Humans
Gray Matter
Aged
Aged, 80 and over
Neurons
biology
Multiple sclerosis
Organ Size
Middle Aged
medicine.disease
White Matter
Myelin basic protein
030104 developmental biology
medicine.anatomical_structure
nervous system
Neurology
Brain size
biology.protein
Female
Neurology (clinical)
030217 neurology & neurosurgery
Demyelinating Diseases
Subjects
Details
- Language :
- English
- ISSN :
- 03051846
- Database :
- OpenAIRE
- Journal :
- Carassiti, D, Altmann, D R, Petrova, N, Pakkenberg, B, Scaravilli, F & Schmierer, K 2018, ' Neuronal loss, demyelination and volume change in the multiple sclerosis neocortex ', Neuropathology and Applied Neurobiology, vol. 44, no. 4, pp. 377-390 . https://doi.org/10.1111/nan.12405
- Accession number :
- edsair.doi.dedup.....a17647211774e4ef3e734f785c79371a