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Implications of epigallocatechin-3-gallate in cultured human Sertoli cells glycolytic and oxidative profile
- Source :
- Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos), Agência para a Sociedade do Conhecimento (UMIC)-FCT-Sociedade da Informação, instacron:RCAAP
- Publication Year :
- 2017
-
Abstract
- Sertoli cells are crucial for the success of spermatogenesis, which is the biological process that ensures male fertility. These cells present high metabolic rates, being often subjected to high oxidative stress levels that, if uncontrolled, may compromise male fertility. Since the most abundant tea catechin, epigallocatechin-3-gallate (EGCG), has demonstrated a potent preventive activity against oxidative stress, we have evaluated its effect at concentrations of 5 and 50μM, on the metabolism, mitochondrial functionality and oxidative profile of human Sertoli cells (hSCs). While, the highest concentration of EGCG (50μM) increased glucose and pyruvate consumption, it decreased the conversion of pyruvate to alanine to sustain a regular lactate production. However, despite maintaining Krebs cycle functionality, EGCG (50μM) decreased mitochondrial membrane potential of hSCs, which could compromise the normal rates of ATP production. Interestingly, oxidative damages to proteins and lipids decreased in this experimental group, which may be valuable for the nutritional support of spermatogenesis.
- Subjects :
- 0301 basic medicine
Male
Monocarboxylic Acid Transporters
medicine.medical_specialty
Muscle Proteins
Oxidative phosphorylation
Carbohydrate metabolism
Biology
Toxicology
medicine.disease_cause
Catechin
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Internal medicine
Pyruvic Acid
medicine
Humans
Glycolysis
Membrane Potential Mitochondrial
Cells, Cultured
Cell Proliferation
Membrane Potential, Mitochondrial
Glucose Transporter Type 2
Alanine
Sertoli Cells
L-Lactate Dehydrogenase
General Medicine
Metabolism
Sertoli cell
Citric acid cycle
Oxidative Stress
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Glucose
chemistry
030220 oncology & carcinogenesis
Pyruvic acid
Cells Cultured
Oxidative stress
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos), Agência para a Sociedade do Conhecimento (UMIC)-FCT-Sociedade da Informação, instacron:RCAAP
- Accession number :
- edsair.doi.dedup.....a166f8fb37839f6aaf84c895d4ecc489