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KAI1 reverses the epithelial-mesenchymal transition in human pancreatic cancer cells
- Source :
- Hepatobiliary & Pancreatic Diseases International. 18:471-477
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Epithelial-mesenchymal transition (EMT) plays an important role in pancreatic cancer (PC). In the present study, we investigated the effects of KAI1 gene overexpression on the EMT of human PC cell lines, MIA PaCa-2 and PACN-1.Plasmids overexpressing KAI1 and pCMV were transfected into MIA PaCa-2 and PACN-1 cells, respectively. After selection of differently transfected cells by G418, KAI1 protein levels were examined by Western blotting, and transfected cells were renamed as MIA PaCa-2-K, MIA PaCa-2-p, PACN-1-K and PACN-1-p. Wound healing and Transwell migration assays were then performed comparing the two groups of cells. EMT-related markers were analyzed by Western blotting.The percentage of wound closure significantly decreased in MIA PaCa-2-K cells compared with MIA PaCa-2-p and MIA PaCa-2 cells after 24, 48 and 72 h (P 0.05). In PACN-1-K cells, the percentage of wound closure significantly decreased as well (P 0.05). Numbers of invading MIA PaCa-2, MIA PaCa-2-p and MIA PaCa-2-K cells were determined as 48.0 ± 15.4, 50.0 ± 12.4, and 12.0 ± 3.8, respectively. The corresponding numbers of invading PACN-1, PACN-1-p and PACN-1-K cells were 29.0 ± 10.6, 31.0 ± 11.4, and 8.0 ± 4.2, respectively. KAI1 overexpression induced a significant upregulation of E-cadherin and also significant downregulation of Snail, vimentin, matrix metalloproteinase 2 (MMP2) and MMP9 (all P 0.05) in PC cells.KAI1 reversed EMT-related marker expression and inhibited migration and invasion of PC cells. Thus, KAI1 might represent a novel potential therapeutic target for PC.
- Subjects :
- Epithelial-Mesenchymal Transition
endocrine system diseases
Gene Expression
Kangai-1 Protein
Transfection
03 medical and health sciences
0302 clinical medicine
Cell Movement
Cell Line, Tumor
Pancreatic cancer
medicine
Humans
Vimentin
Epithelial–mesenchymal transition
Gene
Wound Healing
Migration Assay
Hepatology
business.industry
Gastroenterology
Cadherins
medicine.disease
digestive system diseases
Pancreatic Neoplasms
Blot
Matrix Metalloproteinase 9
Cell culture
030220 oncology & carcinogenesis
embryonic structures
Cancer research
Matrix Metalloproteinase 2
030211 gastroenterology & hepatology
Snail Family Transcription Factors
Wound healing
business
Subjects
Details
- ISSN :
- 14993872
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Hepatobiliary & Pancreatic Diseases International
- Accession number :
- edsair.doi.dedup.....a14975082ea67d109999a0fc16886be1