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Isolation and identification of mannose-binding proteins and estimation of their abundance in sera from hepatocellular carcinoma patients
- Source :
- PROTEOMICS. 13:878-892
- Publication Year :
- 2013
- Publisher :
- Wiley, 2013.
-
Abstract
- The interaction of glycan-binding proteins (GBPs) and glycans plays a significant biological role that ranges from cell-cell recognition to cell trafficking, and glycoprotein targeting. The anomalies of GBPs related to the types and/or quantities were not clearly known in cancer incidence. It is imperative to identify and annotate the GBPs related with the canceration. Here the mannose-binding proteins (MBPs) from the clinical sera were isolated and identified by the mannose-magnetic particle conjugates and the high-accuracy MS analysis. Seventy-five MBPs from normal donors' sera and 79 MBPs from hepatocellular carcinoma patients' sera were identified and annotated. By using the stringent criteria of exponentially modified protein abundance index (emPAI) quantification, 12 MBPs were estimated to be significantly upregulated (emPAI ratio > 4) and nine MBPs were estimated to be significantly downregulated (emPAI ratio < 0.25) in the hepatocellular carcinoma sera. Real-time quantitative PCR, Western blotting, and protein microarrays were also used to confirm the altered MBPs expression level and the specific binding between the isolated MBPs and mannose. The sequence recognition motifs and structure preference of the isolated MBPs were characterized. The functional enrichment analysis revealed that over 57% of the isolated MBPs were binding protein and the upregulated MBPs were involved in cell death, tumor progression, and macromolecular complex remodeling.
- Subjects :
- Carcinoma, Hepatocellular
Amino Acid Motifs
Blotting, Western
Molecular Sequence Data
Protein Array Analysis
Mannose
Biology
Real-Time Polymerase Chain Reaction
Biochemistry
Macromolecular complex remodeling
chemistry.chemical_compound
Tandem Mass Spectrometry
Humans
Amino Acid Sequence
Molecular Biology
Peptide sequence
chemistry.chemical_classification
Binding protein
Mannose binding
Liver Neoplasms
Molecular biology
Neoplasm Proteins
Blot
Mannose-Binding Lectins
chemistry
Case-Control Studies
Protein microarray
Glycoprotein
Chromatography, Liquid
Subjects
Details
- ISSN :
- 16159853
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- PROTEOMICS
- Accession number :
- edsair.doi.dedup.....a13c5463cedd00ea8d931af16ed13564