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Characterization of rabbit κ-casein cDNA: control of κ-casein gene expression in vivo and in vitro
- Source :
- Journal of Molecular Endocrinology, Journal of Molecular Endocrinology, BioScientifica, 1993, 11, pp.9-17
- Publication Year :
- 1993
- Publisher :
- Bioscientifica, 1993.
-
Abstract
- The rabbit κ-casein cDNA was cloned and sequenced. One of the isolated clones included almost the entire 5′ end, while another clone corresponded to the 3′ end of the cDNA. No polyadenylation site was found and therefore this clone did not harbour the complete cDNA. The amino acid sequence of a full-length protein was deduced from the nucleotide sequence obtained for this partial cDNA. It revealed the presence of a chymosin cleavage site and five potential phosphorylation sites. Rabbit κ-casein was compared with those already described in other species. The rabbit sequence is closer to the ovine than to the mouse sequence. This result supports the idea that Lagomorpha are not closer to Rodentia than to Artiodactyla. The cDNA described above was used to study κ-casein gene expression in the rabbit mammary gland. This expression was induced primarily by prolactin in mammary gland organoids and was similar to αs1-casein gene expression in vivo. The κ-casein gene present in the casein gene locus is thus subject to the same regulation as the αs1-casein gene, although it has evolved from a fibrinogen gene.
- Subjects :
- DNA, Complementary
[SDV]Life Sciences [q-bio]
Molecular Sequence Data
Molecular cloning
Biology
Polymerase Chain Reaction
Mammary Glands, Animal
Endocrinology
Pregnancy
Complementary DNA
Casein
Gene expression
Animals
Lactation
Amino Acid Sequence
RNA, Messenger
Northern blot
Molecular Biology
Peptide sequence
Gene
Cells, Cultured
ComputingMilieux_MISCELLANEOUS
DNA Primers
Gene Library
Binding Sites
Base Sequence
Nucleic acid sequence
Caseins
Blotting, Northern
Molecular biology
Prolactin
[SDV] Life Sciences [q-bio]
Gene Expression Regulation
Female
Rabbits
Oligonucleotide Probes
Chymosin
Subjects
Details
- ISSN :
- 14796813 and 09525041
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Journal of Molecular Endocrinology
- Accession number :
- edsair.doi.dedup.....a13827217c64080b19b26dcff3e1e24f