Back to Search Start Over

High Dimensional Imaging Mass Cytometry Panel to Visualize the Tumor Immune Microenvironment Contexture

Authors :
Roxane Elaldi
Patrice Hemon
Luciana Petti
Estelle Cosson
Belinda Desrues
Anne Sudaka
Gilles Poissonnet
Ellen Van Obberghen-Schilling
Jacques-Olivier Pers
Veronique M. Braud
Fabienne Anjuère
Aïda Meghraoui-Kheddar
Institut de pharmacologie moléculaire et cellulaire (IPMC)
Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS)
COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)
Lymphocyte B et Auto-immunité (LBAI)
Université de Brest (UBO)-Institut Brestois Santé Agro Matière (IBSAM)
Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Institut de Biologie Valrose (IBV)
Université Nice Sophia Antipolis (... - 2019) (UNS)
COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)
Centre Antoine Lacassagne, Nice, France
ANR-19-P3IA-0002,3IA@cote d'azur,3IA Côte d'Azur(2019)
BRAUD, Veronique
3IA Côte d'Azur - - 3IA@cote d'azur2019 - ANR-19-P3IA-0002 - P3IA - VALID
Université Nice Sophia Antipolis (1965 - 2019) (UNS)
COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)
Lymphocytes B, Autoimmunité et Immunothérapies (LBAI)
Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-LabEX IGO Immunothérapie Grand Ouest
Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Institut Brestois Santé Agro Matière (IBSAM)
Université de Brest (UBO)
COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)
Source :
Frontiers in Immunology, Frontiers in Immunology, Frontiers, 2021, 12, ⟨10.3389/fimmu.2021.666233⟩, Frontiers in Immunology, 2021, 12, ⟨10.3389/fimmu.2021.666233⟩, Frontiers in Immunology, Vol 12 (2021)
Publication Year :
2021
Publisher :
Frontiers Media SA, 2021.

Abstract

The integrative analysis of tumor immune microenvironment (TiME) components, their interactions and their microanatomical distribution is mandatory to better understand tumor progression. Imaging Mass Cytometry (IMC) is a high dimensional tissue imaging system which allows the comprehensive and multiparametric in situ exploration of tumor microenvironments at a single cell level. We describe here the design of a 39-antibody IMC panel for the staining of formalin-fixed paraffin-embedded human tumor sections. We also provide an optimized staining procedure and details of the experimental workflow. This panel deciphers the nature of immune cells, their functions and their interactions with tumor cells and cancer-associated fibroblasts as well as with other TiME structural components known to be associated with tumor progression like nerve fibers and tumor extracellular matrix proteins. This panel represents a valuable innovative and powerful tool for fundamental and clinical studies that could be used for the identification of prognostic biomarkers and mechanisms of resistance to current immunotherapies.

Details

ISSN :
16643224
Volume :
12
Database :
OpenAIRE
Journal :
Frontiers in Immunology
Accession number :
edsair.doi.dedup.....a136cc1b49cf63e44c246fa151d61c13
Full Text :
https://doi.org/10.3389/fimmu.2021.666233