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High Dimensional Imaging Mass Cytometry Panel to Visualize the Tumor Immune Microenvironment Contexture
- Source :
- Frontiers in Immunology, Frontiers in Immunology, Frontiers, 2021, 12, ⟨10.3389/fimmu.2021.666233⟩, Frontiers in Immunology, 2021, 12, ⟨10.3389/fimmu.2021.666233⟩, Frontiers in Immunology, Vol 12 (2021)
- Publication Year :
- 2021
- Publisher :
- Frontiers Media SA, 2021.
-
Abstract
- The integrative analysis of tumor immune microenvironment (TiME) components, their interactions and their microanatomical distribution is mandatory to better understand tumor progression. Imaging Mass Cytometry (IMC) is a high dimensional tissue imaging system which allows the comprehensive and multiparametric in situ exploration of tumor microenvironments at a single cell level. We describe here the design of a 39-antibody IMC panel for the staining of formalin-fixed paraffin-embedded human tumor sections. We also provide an optimized staining procedure and details of the experimental workflow. This panel deciphers the nature of immune cells, their functions and their interactions with tumor cells and cancer-associated fibroblasts as well as with other TiME structural components known to be associated with tumor progression like nerve fibers and tumor extracellular matrix proteins. This panel represents a valuable innovative and powerful tool for fundamental and clinical studies that could be used for the identification of prognostic biomarkers and mechanisms of resistance to current immunotherapies.
- Subjects :
- 0301 basic medicine
Skin Neoplasms
Tissue imaging
[SDV]Life Sciences [q-bio]
Immune microenvironment
Immunology
imaging mass cytometry
High dimensional
Workflow
Extracellular matrix
03 medical and health sciences
panel design
0302 clinical medicine
Immune system
Biomarkers, Tumor
Tumor Microenvironment
Methods
Humans
Immunology and Allergy
Mass cytometry
Image Cytometry
tumor immune microenvironment
Tumor microenvironment
Chemistry
biomarkers
RC581-607
Immunohistochemistry
[SDV] Life Sciences [q-bio]
030104 developmental biology
Tumor progression
030220 oncology & carcinogenesis
Carcinoma, Squamous Cell
Disease Progression
Cancer research
immune therapies
Immunologic diseases. Allergy
Subjects
Details
- ISSN :
- 16643224
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Frontiers in Immunology
- Accession number :
- edsair.doi.dedup.....a136cc1b49cf63e44c246fa151d61c13
- Full Text :
- https://doi.org/10.3389/fimmu.2021.666233