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Cerebellar Cytokine Expression in a Rat Model for Fetal Asphyctic Preconditioning and Perinatal Asphyxia
- Source :
- Cerebellum, 13(4), 471-478. Springer, Cerebellum (London, England)
- Publication Year :
- 2014
-
Abstract
- Asphyctic brain injury is a major cause of neuronal inflammation in the perinatal period. Fetal asphyctic preconditioning has been shown to modulate the cerebral inflammatory cytokine response, hereby protecting the brain against asphyctic injury at birth. This study was designated to examine the effects of perinatal asphyxia and fetal asphyctic preconditioning on the inflammatory cytokine response in the cerebellum. Fetal asphyxia was induced at embryonic day 17 by clamping the uterine vasculature for 30 min. At term birth, global perinatal asphyxia was induced by placing the uterine horns in saline for 19 min. Pro- and anti-inflammatory cytokine expression were assessed by real-time PCR and immunohistochemistry in cerebella of newborn rats. We found that tumor necrosis factor alpha and interleukin-10 mRNA were increased 12 h after fetal asphyxia, while the inflammatory cytokine response was decreased 96 h postfetal asphyxia. When applied as preconditioning stimulus, fetal asphyxia attenuates the cerebellar cytokine response. These results indicate that sublethal fetal asphyxia may protect the cerebellum from perinatal asphyxia-induced damage via inhibition of inflammation.
- Subjects :
- Male
Cerebellum
congenital, hereditary, and neonatal diseases and abnormalities
Time Factors
Inflammation
Biology
Fetal Hypoxia
Rats, Sprague-Dawley
Andrology
Pregnancy
medicine
Animals
RNA, Messenger
Ischemic Preconditioning
reproductive and urinary physiology
Asphyxia
Medicine(all)
Analysis of Variance
Asphyxia Neonatorum
Original Paper
Fetus
Age Factors
Gene Expression Regulation, Developmental
Embryo, Mammalian
medicine.disease
Rats
Perinatal asphyxia
Disease Models, Animal
medicine.anatomical_structure
Animals, Newborn
Neurology
Immunology
Ischemic preconditioning
Cytokines
Rat
Female
Tumor necrosis factor alpha
Neurology (clinical)
Fetal asphyctic preconditioning
medicine.symptom
Subjects
Details
- Language :
- English
- ISSN :
- 14734222
- Volume :
- 13
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Cerebellum
- Accession number :
- edsair.doi.dedup.....a1308de814607e4071e9998b993588dd