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Optimized interferon-gamma release assays for detection of Mycobacterium bovis infection in African buffaloes (Syncerus caffer)
- Source :
- Veterinary Immunology and Immunopathology. 231:110163
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- The African buffalo (Syncerus caffer) is an economically and ecologically important wildlife species in South Africa; it is also a primary wildlife maintenance host of Mycobacterium bovis. Accurate and early detection of M. bovis infection in buffaloes is important for controlling transmission. Assays that detect cell-mediated immune responses to M. bovis in buffaloes have been developed although these often display suboptimal sensitivity or specificity. Therefore, the aim of this study was to evaluate the newly available Mabtech bovine interferon-gamma (IFN-γ) ELISAPRO kit and optimize its use for detection of buffalo IFN-γ in whole blood samples stimulated with the QuantiFERON® TB Gold Plus antigens. Additionally, the test performance of the Mabtech IFN-γ release assay (IGRA) was compared to the currently used Cattletype® IGRA by determining buffalo-specific cut-off values for the two IGRAs and using gold standard-positive (M. bovis culture-confirmed) and M. bovis-unexposed negative cohorts. Validation of the Mabtech ELISA revealed negligible matrix interference and a linear and parallel response for recombinant bovine and native buffalo IFN-γ in the range 1.95–250 pg/mL. Intra- and inter-assay reproducibility produced coefficients of variation
- Subjects :
- Buffaloes
040301 veterinary sciences
Immunology
Interferon gamma release assay
Early detection
Enzyme-Linked Immunosorbent Assay
Biology
Cohort Studies
0403 veterinary science
Interferon-gamma
03 medical and health sciences
Immune system
Antigen
parasitic diseases
Bovine tuberculosis
medicine
Animals
Tuberculosis
Interferon gamma
030304 developmental biology
0303 health sciences
Mycobacterium bovis
General Veterinary
04 agricultural and veterinary sciences
biology.organism_classification
Virology
Test performance
Interferon-gamma Release Tests
medicine.drug
Subjects
Details
- ISSN :
- 01652427
- Volume :
- 231
- Database :
- OpenAIRE
- Journal :
- Veterinary Immunology and Immunopathology
- Accession number :
- edsair.doi.dedup.....a12c2ff11757edac634127653fa62757