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Identification of antifungal compounds produced by Lactobacillus casei AST18
- Source :
- Current microbiology. 65(2)
- Publication Year :
- 2012
-
Abstract
- Lactobacillus casei AST18 was screened as an antifungal lactic acid bacteria which we have reported before. In this research, the antifungal properties of cell-free culture filtrate (CCF) from L. casei AST18 were detected, and the antifungal compounds of CCF were prepared by ultrafiltration, and semi-preparative HPLC, and then determined by GC–MS. CCF was sensitive to pH and heat treatment but it was not affected by the treatment of trypsin and pepsin. Through the treatment of ultrafiltration and semi-preparative HPLC there were two parts of CCF which showed antifungal activities: part 1 and part 4. Lactic acid was identified as the main antifungal compound in part 1. In part 4, three small molecular substances were detected with GC–MS. The three potential antifungal substances were cyclo-(Leu-Pro), 2,6-diphenyl-piperidine, and 5,10-diethoxy-2,3,7,8-tetrahydro-1H,6H-dipyrrolo[1,2-a;1′,2′-d]pyrazine. The antifungal activity of L. casei AST18 was a synergistic effect of lactic acid and cyclopeptides.
- Subjects :
- Lactobacillus casei
Antifungal Agents
Ultrafiltration
Applied Microbiology and Biotechnology
Microbiology
High-performance liquid chromatography
Peptides, Cyclic
Gas Chromatography-Mass Spectrometry
chemistry.chemical_compound
Pepsin
Piperidines
medicine
Lactic Acid
Chromatography, High Pressure Liquid
Chromatography
biology
Temperature
food and beverages
General Medicine
Dipeptides
Hydrogen-Ion Concentration
biology.organism_classification
Trypsin
Lactic acid
Culture Media
Lacticaseibacillus casei
chemistry
Biochemistry
Pyrazines
biology.protein
Gas chromatography–mass spectrometry
Bacteria
medicine.drug
Subjects
Details
- ISSN :
- 14320991
- Volume :
- 65
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Current microbiology
- Accession number :
- edsair.doi.dedup.....a0f991fde4bc29f922b4a3698df82880