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Anti-senescence and anti-wrinkle activities of 3—bromo—4,5—dihydroxybenzaldehyde from Polysiphonia morrowii Harvey in human dermal fibroblasts
- Source :
- Asian Pacific Journal of Tropical Biomedicine, Vol 11, Iss 2, Pp 74-80 (2021)
- Publication Year :
- 2021
- Publisher :
- Wolters Kluwer Medknow Publications, 2021.
-
Abstract
- Objective: To investigate the anti-senescence effect of 3-bromo-4,5- dihydroxybenzaldehyde (BDB) from Polysiphonia morrowii Harvey in human dermal fibroblasts (HDF). Methods: HDF were subjected to treatment of BDB and then treated with hydrogen peroxide (H2O2) to induce premature senescence. Senescence-associated β-galactosidase (SA-β-gal) activity in HDF was determined using the SA-β-gal staining method. Intracellular reactive oxygen species (ROS) production was measured using the 2’,7’-dichlorodihydrofluorescein diacetate assay. Western blotting assay was performed to assess the level of antioxidant enzyme glutathione peroxidase 1 (GPX1). In addition, intracellular collagen and collagenase contents were analyzed using the respective ELISA kits. Elastase activity in HDF supernatants was measured from p-nitroaniline release and normalized using total protein content. Results: Treatment of HDF with H2O2 increased the activity of SAP-gal, but BDB pre-treatment resulted in the reduction of SA-β- gal activity. Moreover, BDB significantly reduced H2O2-induced intracellular ROS production. BDB also markedly increased the level of GPX1, which was inhibited by 400 µM of H2O2. Furthermore, in in vitro study, BDB significantly increased intracellular collagen content and decreased matrix metalloproteinase-1 and elastase activities in HDF. Conclusions: Our results demonstrate that BDB shows anti- senescence and anti-wrinkle activities in vitro.
- Subjects :
- GPX1
Antioxidant
polysiphonia morrowii harvey
3-bromo-4
lcsh:Arctic medicine. Tropical medicine
integumentary system
Chemistry
lcsh:RC955-962
medicine.medical_treatment
Elastase
medicine.disease_cause
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Molecular biology
In vitro
Blot
body regions
lcsh:Biology (General)
5- dihydroxybenzaldehyde
oxidative stress
human dermal fibroblast
anti-senescence activity
anti-wrinkle activity
medicine
Collagenase
lcsh:QH301-705.5
Oxidative stress
Intracellular
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 25889222 and 22211691
- Volume :
- 11
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Asian Pacific Journal of Tropical Biomedicine
- Accession number :
- edsair.doi.dedup.....a0ef6ed856d8006ab23f68f893b9c27e