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Identification and Full Characterisation of Two Novel Crustacean Infecting Members of the Family Nudiviridae Provides Support for Two Subfamilies

Authors :
Monique M. van Oers
Clauvis Nji Tizi Taning
Grant D. Stentiford
Rose Kerr
Guy Smagghe
Benigna Van Eynde
Olivier Christiaens
Chantelle Hooper
Kelly S. Bateman
Jamie Bojko
Tim P. Bean
Daan Delbare
Ronny van Aerle
Source :
Viruses, Vol 13, Iss 1694, p 1694 (2021), Bateman, K S, Kerr, R, Stentiford, G D, Bean, T, Hooper, C, Van Eynde, B, Delbare, D, Bojko, J, Christiaens, O, Taning, C N T, Smagghe, G, Van Oers, M M & van Aerle, R 2021, ' Identification and full characterisation of two novel crustacean infecting members of the family Nudiviridae provides support for two subfamilies ', Viruses, vol. 13, no. 9 . https://doi.org/10.3390/v13091694, Viruses 13 (2021) 9, Viruses, 13(9), VIRUSES-BASEL, Viruses, Volume 13, Issue 9
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

Multiple enveloped viruses with rod-shaped nucleocapsids have been described, infecting the epithelial cell nuclei within the hepatopancreas tubules of crustaceans. These bacilliform viruses share the ultrastructural characteristics of nudiviruses, a specific clade of viruses infecting arthropods. Using histology, electron microscopy and high throughput sequencing, we characterise two further bacilliform viruses from aquatic hosts, the brown shrimp (Crangon crangon) and the European shore crab (Carcinus maenas). We assembled the full double stranded, circular DNA genome sequences of these viruses (~113 and 132 kbp, respectively). Comparative genomics and phylogenetic analyses confirm that both belong within the family Nudiviridae but in separate clades representing nudiviruses found in freshwater and marine environments. We show that the three thymidine kinase (tk) genes present in all sequenced nudivirus genomes, thus far, were absent in the Crangon crangon nudivirus, suggesting there are twenty-eight core genes shared by all nudiviruses. Furthermore, the phylogenetic data no longer support the subdivision of the family Nudiviridae into four genera (Alphanudivirus to Deltanudivirus), as recently adopted by the International Committee on Taxonomy of Viruses (ICTV), but rather shows two main branches of the family that are further subdivided. Our data support a recent proposal to create two subfamilies within the family Nudiviridae, each subdivided into several genera.

Details

Language :
English
ISSN :
19994915
Volume :
13
Issue :
1694
Database :
OpenAIRE
Journal :
Viruses
Accession number :
edsair.doi.dedup.....a0ebc5a5e45427119097e9e8b211af05