Succinate semialdehyde dehydrogenase deficiency does not down-regulate γ-hydroxybutyric acid binding sites in the mouse brain

We investigated whether succinate semialdehyde dehydrogenase deficiency alters γ-hydroxybutyric acid (GHB) receptor characteristics due to elevation of GHB levels in the mouse brain. The membrane homogenate binding and quantitative autoradiography using [ 3 H]NCS-382 revealed no significant changes in the affinity ( K d ), receptor density ( B max ), or displacement potency (IC 50 ) in various brain regions of Aldh5a1 −/− vs. Aldh5a1 +/+ mice.