Chemiluminiscence sensor for high-throughput DNA sequencing

The design and development of an inexpensive and customizable platform, for ultra-high throughput pyrosequenicng is presented. The platform comprises a micro-chip containing millions of reaction wells on a fiber optic platform, integrated with fluidics and a CMOS image sensor for chemiluminescence detection. Proof of concept experiments were performed to demonstrate the viability of the system for sequencing. Synthetic DNA, with various sequences, were designed and sequenced in the Pyrome sequencer. An experiment designed to test the loss of beads during long reads demonstrated that there is no appreciable loss of beads after sequencing 25 base pairs.