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Molecular Weight Analysis of Blue Shark (Prionace glauca) Collagen Hydrolysates by GPC-LS; Effect of High Molecular Weight Hydrolysates on Fibroblast Cultures: mRNA Collagen Type I Expression and Synthesis
- Source :
- International Journal of Molecular Sciences; Volume 23; Issue 1; Pages: 32, Digital.CSIC. Repositorio Institucional del CSIC, instname, International Journal of Molecular Sciences, Vol 23, Iss 32, p 32 (2022)
- Publication Year :
- 2022
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2022.
-
Abstract
- 14 pages, 8 figures, 4 tables.-- This is an open access article distributed under the Creative Commons Attribution License<br />High molecular weight (Mw) collagen hydrolysates have been demonstrated to produce a higher synthesis of collagen type I mRNA. Mw determination is a key factor maximizing the effect of collagen hydrolysates on collagen type I synthesis by fibroblasts. This work aimed to achieve a high average Mw in Blue Shark Collagen Hydrolysate, studying different hydrolysis parameters by GPC-LS analysis and testing its effect on mRNA Type I collagen expression. Analysis revealed differences in blue shark collagen hydrolysates Mw depending on hydrolysis conditions. Papain leads to obtaining a significantly higher Mw hydrolysate than Alcalase at different times of hydrolysis and at different enzyme/substrate ratios. Besides, the time of the hydrolysis factor is more determinant than the enzyme/substrate ratio factor for obtaining a higher or lower hydrolysate Mw when using Papain as the enzyme. Contrary, Alcalase hydrolysates resulted in similar Mw with no significant differences between different conditions of hydrolysis assayed. Blue shark collagen hydrolysate showing the highest Mw showed neither cytotoxic nor proliferation effect on fibroblast cell culture. Besides, it exhibited an increasing effect on both mRNA expression and pro-collagen I production<br />This research was funded by the project BlueHuman, EU_EAPA_151/2016 and Xunta de Galicia (Grupos de Potencial Crecimiento, IN607B2021/11)
- Subjects :
- QH301-705.5
Fish by-products
Organic Chemistry
GPC-LS
General Medicine
Molecular weight
Catalysis
Computer Science Applications
mRNA collagen expression
Inorganic Chemistry
Chemistry
Fish protein hydrolysates
Ppro-collagen
Fibroblast
Collagen
Biology (General)
Physical and Theoretical Chemistry
fish by-products
collagen
fish protein hydrolysates
molecular weight
fibroblast
pro-collagen
QD1-999
Molecular Biology
Spectroscopy
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences; Volume 23; Issue 1; Pages: 32, Digital.CSIC. Repositorio Institucional del CSIC, instname, International Journal of Molecular Sciences, Vol 23, Iss 32, p 32 (2022)
- Accession number :
- edsair.doi.dedup.....a0c8acf649bf60e9f692ed201347b5e1