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Dietary multivalent anti‐ Helicobacter pylori immunoglobulin Y significantly increase the H. pylori eradication and improve the clinical symptoms in patients
- Source :
- Helicobacter. 26
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- BACKGROUND The oral chicken immunoglobulin Y (IgY) as a novel model of immunotherapy to control Helicobacter pylori (H. pylori, Hp) infection has gained much interest in recent years. However, none of the current IgY therapies showed a total eradication of H. pylori on patients. METHODS In this report, the recombinant antigens of H. pylori, including UreB (1710 bp), BabA2 (1269 bp), and FlaA (399 bp), were, respectively, expressed and purified, and then mixed and subjected to immunize laying hens for the preparation of multivalent anti-H. pylori immunoglobulin Y (anti-Hp mIgY). Next, the biological activities of anti-Hp mIgY, including the recognition to antigens and the inhibition on H. pylori growth, were tested. Moreover, to perform a clinical trial, 94 Hp-infected patients, according to the values of 13 C urea breath test and the characteristics of gastroscopy of volunteers, were enrolled to evaluate the effects of dietary anti-Hp mIgY against H. pylori infection. After continuous dietary of anti-Hp mIgY for 2 weeks, the oral administration was terminated. The clinical symptoms of the patients were followed up at 2nd, 4th, and 6th week, respectively, and the 13 C urea breath test were re-examined at 6th week. RESULTS The anti-Hp mIgY could bind to recombinant antigens very well, and the titers of anti-Hp mIgY to UreB, Baba2, and FlaA, are 62.5, 125, and 250 μg/ml, respectively. The in vitro antibacterial test showed that the 2 mg/ml of anti-Hp mIgY could completely inhibit the H. pylori growth for 36 h. After a 2-week dietary of anti-Hp mIgY, the value of 13 C urea breath test was significantly decreased by 56.0% (25.9 ± 14.1 vs 11.4 ± 9.78, p
- Subjects :
- medicine.medical_specialty
medicine.medical_treatment
Urea breath test
Immunoglobulins
Gastroenterology
Helicobacter Infections
Antigen
Oral administration
Internal medicine
medicine
Animals
Humans
Helicobacter pylori
biology
medicine.diagnostic_test
business.industry
General Medicine
Immunotherapy
biology.organism_classification
Clinical trial
Titer
Infectious Diseases
Breath Tests
Quality of Life
biology.protein
Immunoglobulin Y
Female
business
Chickens
Subjects
Details
- ISSN :
- 15235378 and 10834389
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Helicobacter
- Accession number :
- edsair.doi.dedup.....a0be801c14ade2176154aa6d5f61674a