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Exercising Spatiotemporal Control of Cell Attachment with Optically Transparent Microelectrodes
- Source :
- Langmuir. 24:6837-6844
- Publication Year :
- 2008
- Publisher :
- American Chemical Society (ACS), 2008.
-
Abstract
- This paper describes a novel approach of controlling cell-surface interactions through an electrochemical “switching” of biointerfacial properties of optically transparent microelectrodes. The indium tin oxide (ITO) microelectrodes, fabricated on glass substrates, were modified with poly(ethylene glycol) (PEG) silane to make glass and ITO regions resistant to protein and cell adhesion. Cyclic voltammetry, with potassium ferricyanide serving as a redox reporter molecule, was used to monitor electron transfer across the electrolyte–ITO interface. PEG silane modification of ITO correlated with diminished electron transfer, judged by the disappearance of ferricyanide redox activity. Importantly, application of reductive potential (−1.4 V vs Ag/AgCl reference) corresponded with reappearance of typical ferricyanide redox peaks, thus pointing to desorption of an insulating PEG silane layer. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) characterization of the silanized ITO surfaces after electrical stimulation indicated complete removal of the silane layer. Significantly, electrical stimulation allowed to “switch” chosen electrodes from nonfouling to protein-adhesive while leaving other ITO and glass regions protected by a nonfouling PEG silane layer. The spatial and temporal control of biointerfacial properties afforded by our approach was utilized to micropattern proteins and cells and to construct micropatterned co-cultures. In the future, control of the biointerfacial properties afforded by this novel approach may allow the organization of multiple cell types into precise geometric configurations in order to create better in vitro mimics of cellular complexity of the native tissues.
- Subjects :
- Molecular Structure
Inorganic chemistry
Cell Culture Techniques
Surfaces and Interfaces
Condensed Matter Physics
Silane
Mass Spectrometry
Article
Indium tin oxide
chemistry.chemical_compound
Microelectrode
Potassium ferricyanide
Electron transfer
chemistry
Chemical engineering
Cell Line, Tumor
Electrode
Cell Adhesion
Electrochemistry
Humans
General Materials Science
Ferricyanide
Cyclic voltammetry
Microelectrodes
Spectroscopy
Subjects
Details
- ISSN :
- 15205827 and 07437463
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Langmuir
- Accession number :
- edsair.doi.dedup.....a0be1411f195f026c5977575af4d0d0e