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Investigation of molecular mechanisms of experimental compounds in murine models of chronic allergic airways disease using synchrotron Fourier-transform infrared microspectroscopy
- Source :
- Scientific Reports, Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
- Publication Year :
- 2019
-
Abstract
- The ovalbumin-induced (OVA) chronic allergic airways murine model is a well-established model for investigating pre-clinical therapies for chronic allergic airways diseases, such as asthma. Here, we examined the effects of several experimental compounds with potential anti-asthmatic effects including resveratrol (RV), relaxin (RLN), l-sulforaphane (LSF), valproic acid (VPA), and trichostatin A (TSA) using both a prevention and reversal model of chronic allergic airways disease. We undertook a novel analytical approach using focal plane array (FPA) and synchrotron Fourier-transform infrared (S-FTIR) microspectroscopic techniques to provide new insights into the mechanisms of action of these experimental compounds. Apart from the typical biological effects, S-FTIR microspectroscopy was able to detect changes in nucleic acids and protein acetylation. Further, we validated the reduction in collagen deposition induced by each experimental compound evaluated. Although this has previously been observed with conventional histological methods, the S-FTIR technique has the advantage of allowing identification of the type of collagen present. More generally, our findings highlight the potential utility of S-FTIR and FPA-FTIR imaging techniques in enabling a better mechanistic understanding of novel asthma therapeutics.
- Subjects :
- 0301 basic medicine
Drug Evaluation, Preclinical
Resveratrol
Hydroxamic Acids
Anti-asthmatic Agent
chemistry.chemical_compound
Mice
0302 clinical medicine
Isothiocyanates
Spectroscopy, Fourier Transform Infrared
Anti-Asthmatic Agents
Infrared spectroscopy
Mice, Inbred BALB C
Multidisciplinary
biology
Chemistry
Histone deacetylase inhibitor
Relaxin
Treatment Outcome
030220 oncology & carcinogenesis
Sulfoxides
Medicine
lipids (amino acids, peptides, and proteins)
Female
medicine.symptom
medicine.drug
medicine.drug_class
Ovalbumin
Science
Inflammation
Article
03 medical and health sciences
medicine
Animals
Asthma
Valproic Acid
Mesenchymal stem cell
medicine.disease
Disease Models, Animal
030104 developmental biology
Trichostatin A
Chronic Disease
Cancer research
biology.protein
Synchrotrons
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....a0ba5c74e74b69f4b367e729fb141757