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Induction of wall ingrowths of transfer cells occurs rapidly and depends upon gene expression in cotyledons of developing Vicia faba seeds
- Source :
- Protoplasma. 231:15-23
- Publication Year :
- 2007
- Publisher :
- Springer Science and Business Media LLC, 2007.
-
Abstract
- Abaxial epidermal cells of developing faba bean (Vicia faba) cotyledons are modified to a transfer cell morphology and function. In contrast, the adaxial epidermal cells do not form transfer cells but can be induced to do so when excised cotyledons are cultured on an agar medium. The first fenestrated layer of wall ingrowths is apparent within 24 h of cotyledon exposure to culture medium. The time course of wall ingrowth formation was examined further. By 2 h following cotyledon excision, a 350 nm thick wall was deposited evenly over the outer periclinal walls of adaxial epidermal cells and densities of cytoplasmic vesicles increased. After 3 h in culture, 10% of epidermal cells contained small projections of wall material on their outer periclinal walls. Thereafter, this percentage rose sharply and reached a maximum of 90% by 15 h. Continuous culture of cotyledons on a medium containing 6-methyl purine (an inhibitor of RNA synthesis) completely blocked wall ingrowth formation. In contrast, if exposure to 6-methyl purine was delayed for 1 h at the start of the culture period, the adaxial epidermal cells were found to contain small wall ingrowths. Treating cotyledons for 1 h with 6-methyl purine at 15 h following cotyledon excision halted further wall ingrowth development. We conclude that transfer cell induction is rapid and that signalling and early events leading to wall ingrowth formation depend upon gene expression. In addition, these gene products have a high turnover rate.
- Subjects :
- Purine
Cytoplasm
Time Factors
food.ingredient
Morphology (linguistics)
Plant Science
Biology
Plant Epidermis
Agar plate
chemistry.chemical_compound
food
Cell Wall
Gene Expression Regulation, Plant
Botany
Gene expression
food and beverages
RNA
Transfer cell
Cell Biology
General Medicine
Vicia faba
Cell biology
chemistry
Seeds
Cotyledon
Subjects
Details
- ISSN :
- 16156102 and 0033183X
- Volume :
- 231
- Database :
- OpenAIRE
- Journal :
- Protoplasma
- Accession number :
- edsair.doi.dedup.....a0a8e1421a95fae71f0dec2cb707ceec