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Whole-Brain Three-Dimensional Profiling Reveals Brain Region Specific Axon Vulnerability in 5xFAD Mouse Model
- Source :
- Frontiers in Neuroanatomy, Vol 14 (2020), Frontiers in Neuroanatomy
- Publication Year :
- 2020
- Publisher :
- Frontiers Media S.A., 2020.
-
Abstract
- Axonopathy is a pathological feature observed in both Alzheimer’s disease (AD) patients and animal models. However, identifying the temporal and regional progression of axonopathy during AD development remains elusive. Using the fluorescence micro-optical sectioning tomography system, we acquired whole-brain datasets in the early stage of 5xFAD/Thy1-GFP-M mice. We reported that among GFP labeled axons, GFP-positive axonopathy first formed in the lateral septal nucleus, subiculum, and medial mammillary nucleus. The axonopathy further increased in most brain regions during aging. However, most of the axonopathic varicosities disappeared significantly in the medial mammillary nucleus after 8 weeks old. Continuous three-dimensional datasets showed that axonopathy in the medial mammillary nucleus was mainly located on axons from hippocampal GFP-positive neurons. Using the rabies viral tracer in combination with immunohistochemistry, we found that axons in the medial mammillary nucleus from the subiculum were susceptible to lesions that prior to the occurrence of behavioral disorders. In conclusion, we created an early-stage spatiotemporal map of axonopathy in 5xFAD/Thy1-GFP-M mice and identified specific neural circuits which are vulnerable to axon lesions in an AD mouse model. These findings underline the importance of early interventions for AD, and may contribute to the understanding of its progression and its early symptom treatment.
- Subjects :
- 0301 basic medicine
Neuroscience (miscellaneous)
three-dimension
Biology
Hippocampal formation
whole-brain imaging
Medial mammillary nucleus
medial mammillary nucleus
lcsh:RC321-571
lcsh:QM1-695
03 medical and health sciences
Cellular and Molecular Neuroscience
0302 clinical medicine
Biological neural network
medicine
Axon
Pathological
lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry
Original Research
Subiculum
lcsh:Human anatomy
early stage
Neuroanatomy
Brain region
axonopathy
030104 developmental biology
medicine.anatomical_structure
Immunohistochemistry
Anatomy
Neuroscience
Alzheimer’s disease
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 16625129
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Frontiers in Neuroanatomy
- Accession number :
- edsair.doi.dedup.....a09d6f85edc435a222f196431da7030e
- Full Text :
- https://doi.org/10.3389/fnana.2020.608177/full