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Transcriptome-wide deregulation of gene expression by artificial light at night in tadpoles of common toads
- Source :
- Science of the Total Environment, Science of the Total Environment, 2022, 818, pp.151734. ⟨10.1016/j.scitotenv.2021.151734⟩, Science of the Total Environment, Elsevier, In press, pp.151734. ⟨10.1016/j.scitotenv.2021.151734⟩
- Publication Year :
- 2022
- Publisher :
- HAL CCSD, 2022.
-
Abstract
- International audience; Artificial light at night (ALAN) affects numerous physiological and behavioural mechanisms in various species by po- tentially disturbing circadian timekeeping systems and modifying melatonin levels. However, given the multiple direct and indirect effects of ALAN on organisms, large-scale transcriptomic approaches are essential to assess the global ef- fect of ALAN on biological processes. Moreover, although studies have focused mainly on variations in gene expression during the night in the presence of ALAN, it is necessary to investigate the effect of ALAN on gene expression during the day. In this study, we combined de novo transcriptome sequencing and assembly, and a controlled laboratory experi- ment to evaluate the transcriptome-wide gene expression response using high-throughput (RNA-seq) in Bufo bufo tad- poles exposed to ecologically relevant light levels. Here, we demonstrated for the first time that ALAN affected gene expression at night (3.5% and 11% of differentially expressed genes when exposed to 0.1 and 5 lx compared to con- trols, respectively), but also during the day (11.2% of differentially expressed genes when exposed to 5 lx compared to controls) with a dose-dependent effect. ALAN globally induced a downregulation of genes (during the night, 58% and 62% of the genes were downregulated when exposed to 0.1 and 5 lx compared to controls, respectively, and dur- ing the day, 61.2% of the genes were downregulated when exposed to 5 lx compared to controls). ALAN effects were detected at very low levels of illuminance (0.1 lx) and affected mainly genes related to the innate immune system and, to a lesser extend to lipid metabolism. These results provide new insights into understanding the effects of ALAN on organism. ALAN impacted the expression of genes linked to a broad range of physiological pathways at very low levels of ALAN during night-time and during daytime, potentially resulting in reduced immune capacity under environmen- tal immune challenges.
- Subjects :
- Environmental Engineering
Light
RNA-Seq
Biology
Bufo bufo
Melatonin
Transcriptome
03 medical and health sciences
0302 clinical medicine
Immune system
Gene expression
medicine
Environmental Chemistry
Animals
Circadian rhythm
Waste Management and Disposal
Gene
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Genetics
0303 health sciences
Innate immune system
Amphibian
Pollution
[SDE.BE] Environmental Sciences/Biodiversity and Ecology
Lipid metabolism
Light pollution
Larva
RNA-seq
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
030217 neurology & neurosurgery
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 00489697 and 18791026
- Database :
- OpenAIRE
- Journal :
- Science of the Total Environment, Science of the Total Environment, 2022, 818, pp.151734. ⟨10.1016/j.scitotenv.2021.151734⟩, Science of the Total Environment, Elsevier, In press, pp.151734. ⟨10.1016/j.scitotenv.2021.151734⟩
- Accession number :
- edsair.doi.dedup.....a084cdad15b7041800835be83e2a6a9e