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Fate of Astrocytes in The Gerbil Hippocampus After Transient Global Cerebral Ischemia
- Source :
- International Journal of Molecular Sciences, Volume 20, Issue 4, International Journal of Molecular Sciences, Vol 20, Iss 4, p 845 (2019)
- Publication Year :
- 2019
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2019.
-
Abstract
- Neuronal death and reactive gliosis are major features of brain tissue damage following transient global cerebral ischemia (tgCI). This study investigated long-term changes in neuronal death and astrogliosis in the gerbil hippocampus for 180 days after 5 min of tgCI. A massive loss of pyramidal neurons was found in the hippocampal CA1 area (CA1) area between 5 and 30 days after tgCI by Fluoro-Jade B (FJB, a marker for neuronal degeneration) histofluorescence staining, but pyramidal neurons in the CA2/3 area did not die. The reaction of astrocytes (astrogliosis) was examined by glial fibrillary acidic protein (GFAP) immunohistochemistry. Morphological change or degeneration (death) of the astrocytes was found in the CA1 area after tgCI, but, in the CA2/3 area, astrogliosis was hardly shown. GFAP immunoreactive astrocytes in the CA1 area was significantly increased in number with time and peaked at 30 days after tgCI, and they began to be degenerated or dead from 40 days after tgCI. The effect was examined by double immunofluorescence staining for FJB and GFAP. The number of FJB/GFAP+ cells (degenerating astrocytes) was gradually increased with time after tgCI. At 180 days after tgCI, FJB/GFAP+ cells were significantly decreased, but FJB+ cells (dead astrocytes) were significantly increased. In brief, 5 min of tgCI induced a progressive degeneration of CA1 pyramidal neurons from 5 until 30 days with an increase of reactive astrocytes, and, thereafter, astrocytes were degenerated with time and dead at later times. This phenomenon might be shown due to the death of neurons.
- Subjects :
- Male
0301 basic medicine
Pathology
medicine.medical_specialty
hippocampus
Fluoro Jade B
Ischemia
Hippocampus
Hippocampal formation
Gerbil
Article
Catalysis
lcsh:Chemistry
Inorganic Chemistry
03 medical and health sciences
0302 clinical medicine
Glial Fibrillary Acidic Protein
medicine
Animals
Cell Lineage
Neuronal degeneration
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
delayed neuronal death
Staining and Labeling
Glial fibrillary acidic protein
biology
Chemistry
Organic Chemistry
General Medicine
transient global ischemia
medicine.disease
Computer Science Applications
Astrogliosis
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
nervous system
Ischemic Attack, Transient
Astrocytes
astrogliosis
biology.protein
Immunohistochemistry
Gerbillinae
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....a06bcc2cf4554c259ea7080ed379394b
- Full Text :
- https://doi.org/10.3390/ijms20040845