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Fate of Astrocytes in The Gerbil Hippocampus After Transient Global Cerebral Ischemia

Authors :
Jae-Chul Lee
Ji Hyeon Ahn
Hyun-Jung Kim
Dae Won Kim
In Koo Hwang
Young-Myeong Kim
Young-Eun Park
Tae-Kyeong Lee
Moo-Ho Won
Hye-Young Kim
Soo Young Choi
Myoung Cheol Shin
Minah Song
Sungwoo Ryoo
Joon Ha Park
Cheolwoo Park
Jun Hwi Cho
Source :
International Journal of Molecular Sciences, Volume 20, Issue 4, International Journal of Molecular Sciences, Vol 20, Iss 4, p 845 (2019)
Publication Year :
2019
Publisher :
Multidisciplinary Digital Publishing Institute, 2019.

Abstract

Neuronal death and reactive gliosis are major features of brain tissue damage following transient global cerebral ischemia (tgCI). This study investigated long-term changes in neuronal death and astrogliosis in the gerbil hippocampus for 180 days after 5 min of tgCI. A massive loss of pyramidal neurons was found in the hippocampal CA1 area (CA1) area between 5 and 30 days after tgCI by Fluoro-Jade B (FJB, a marker for neuronal degeneration) histofluorescence staining, but pyramidal neurons in the CA2/3 area did not die. The reaction of astrocytes (astrogliosis) was examined by glial fibrillary acidic protein (GFAP) immunohistochemistry. Morphological change or degeneration (death) of the astrocytes was found in the CA1 area after tgCI, but, in the CA2/3 area, astrogliosis was hardly shown. GFAP immunoreactive astrocytes in the CA1 area was significantly increased in number with time and peaked at 30 days after tgCI, and they began to be degenerated or dead from 40 days after tgCI. The effect was examined by double immunofluorescence staining for FJB and GFAP. The number of FJB/GFAP+ cells (degenerating astrocytes) was gradually increased with time after tgCI. At 180 days after tgCI, FJB/GFAP+ cells were significantly decreased, but FJB+ cells (dead astrocytes) were significantly increased. In brief, 5 min of tgCI induced a progressive degeneration of CA1 pyramidal neurons from 5 until 30 days with an increase of reactive astrocytes, and, thereafter, astrocytes were degenerated with time and dead at later times. This phenomenon might be shown due to the death of neurons.

Details

Language :
English
ISSN :
14220067
Database :
OpenAIRE
Journal :
International Journal of Molecular Sciences
Accession number :
edsair.doi.dedup.....a06bcc2cf4554c259ea7080ed379394b
Full Text :
https://doi.org/10.3390/ijms20040845