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Effect of Evans Blue and Trypan Blue on Syncytia Formation and Infectivity of Human Immunodeficiency Virus Type I and Type II In Vitro
- Source :
- AIDS Research and Human Retroviruses. 7:537-543
- Publication Year :
- 1991
- Publisher :
- Mary Ann Liebert Inc, 1991.
-
Abstract
- Polyanionic compounds were used to inhibit infectivity of human immunodeficiency virus in vitro. Suramin, Evans blue, and Trypan blue were shown to inhibit syncytia formation normally observed when HIV-1-infected cells are cocultured with CD4+ cells. The inhibition was more pronounced with Evans blue than with any of the other polyanions studied. The inhibitory effect was significantly weaker in HIV-2 systems. However, the reverse transcriptase activities of both types of viruses were inhibited by Evans blue. Another polyanionic compound, phosphorothioate 28-mer cytidine homopolymer (SdC28) was shown to inhibit syncytium formation induced by HIV-1-and HIV-2-infected cells in an identical manner. Evans blue showed partial blocking of gp120 binding to CD4 in a solid-phase enzyme-linked immunosorbent assay (ELISA). These results suggest that the polyanionic dyes may exert their antiviral effects, at least in part, by interfering with the binding and fusion of HIV with susceptible T cells.
- Subjects :
- Suramin
Immunology
Biology
Virus Replication
Antiviral Agents
Virus
Cell Fusion
chemistry.chemical_compound
Virology
Tumor Cells, Cultured
medicine
Humans
Evans Blue
Infectivity
Syncytium
Cell fusion
Molecular Structure
virus diseases
RNA-Directed DNA Polymerase
Trypan Blue
HIV Reverse Transcriptase
In vitro
Infectious Diseases
chemistry
HIV-2
Mutation
HIV-1
Trypan blue
medicine.drug
Subjects
Details
- ISSN :
- 19318405 and 08892229
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- AIDS Research and Human Retroviruses
- Accession number :
- edsair.doi.dedup.....a06a6caad22cf08bbbe1dfe359d6b86b