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Collagen maturity and mineralization in mesenchymal stem cells cultured on the hydroxyapatite-based bone scaffold analyzed by ATR-FTIR spectroscopic imaging
- Source :
- Materials scienceengineering. C, Materials for biological applications. 119
- Publication Year :
- 2020
-
Abstract
- Modern bone tissue engineering is based on the use of implants in the form of biomaterials, which are used as scaffolds for osteoprogenitor or stem cells. The task of the scaffolds is to temporarily sustain the function, proliferation and differentiation of bone tissue to enable its regeneration. The aim of this work is to use the macro ATR-FTIR spectroscopic imaging for analysis of the ceramic-based biomaterial (chitosan/β-1,3-glucan/hydroxyapatite). Specifically, during long-term culture of mesenchymal cells derived from adipose tissue (ADSCs) and bone marrow (BMDSCs) on the surface of scaffold. Infrared spectroscopy allows the acquisition of information on both the organic and inorganic parts of the tested composite. This innovative spectroscopic approach proved to be very suitable for studying the formation of new bone tissue and ECM components, sample staining and demineralization are not required and consequently the approach is rapid and cost-effective. The novelty of this study focuses on the innovatory use of ATR-FTIR imaging to evaluate the molecular structure and maturity of collagen as well as mineral matrix formation and crystallization in the context of bone regenerative medicine. Our research has shown that the biomaterial investigated on this work facilitates the formation of valid bone ECM of the stem cells types studied, as a result of the synthesis of type I collagen and mineral content deposition. Nevertheless, ADSC cells have been proven to produce a greater amount of collagen with a lower content of helical secondary structures, at the same time showing a higher mineralization intensity compared to BMDSC cells. Considering the above results, it could be stated that the developed scaffold is a promising material for biomedical applications, including modification of bone implants to increase their biocompatibility.
- Subjects :
- Scaffold
Materials science
Biocompatibility
Bioengineering
02 engineering and technology
010402 general chemistry
Bone tissue
01 natural sciences
Regenerative medicine
Bone and Bones
Biomaterials
Osteogenesis
Spectroscopy, Fourier Transform Infrared
medicine
Humans
Cells, Cultured
Tissue Engineering
Tissue Scaffolds
Mesenchymal stem cell
Biomaterial
Cell Differentiation
Mesenchymal Stem Cells
021001 nanoscience & nanotechnology
0104 chemical sciences
medicine.anatomical_structure
Durapatite
Mechanics of Materials
Bone marrow
Collagen
0210 nano-technology
Type I collagen
Biomedical engineering
Subjects
Details
- ISSN :
- 18730191
- Volume :
- 119
- Database :
- OpenAIRE
- Journal :
- Materials scienceengineering. C, Materials for biological applications
- Accession number :
- edsair.doi.dedup.....a06371cd6b9f46b1c21c611ccd5132c2