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Acute Plasmodium chabaudi Infection Dampens Humoral Responses to a Secondary T-Dependent Antigen but Enhances Responses to a Secondary T-Independent Antigen
- Source :
- The Journal of Immunology. 191:4731-4739
- Publication Year :
- 2013
- Publisher :
- The American Association of Immunologists, 2013.
-
Abstract
- High rates of coinfection occur in malaria endemic regions, leading to more severe disease outcomes. Understanding how coinfecting pathogens influence the immune system is important in the development of treatment strategies that reduce morbidity and mortality. Using the Plasmodium chabaudi mouse model of malaria and immunization with model Ags that are either T-dependent (4-hydroxy-3-nitrophenyl [NP]-OVA) or T-independent (NP-Ficoll), we analyzed the effects of acute malaria on the development of humoral immunity to secondary Ags. Total Ig and IgG1 NP–specific Ab responses to NP-OVA were significantly decreased in the P. chabaudi–infected group compared with the uninfected group, whereas NP-specific IgG2c Ab was significantly increased in the P. chabaudi–infected group. In contrast, following injection with T-independent NP-Ficoll, the P. chabaudi–infected group had significantly increased NP-specific total Ig, IgM, and IgG2c Ab titers compared with controls. Treatment with anti–IFN-γ led to an abrogation of the NP-specific IgG2c Ab induced by P. chabaudi infection but did not affect other NP-specific Ab isotypes or titers. IFN-γ depletion also increased the percentage of plasma cells in both P. chabaudi–infected and uninfected groups but decreased the percentage of B cells with a germinal center (GC) phenotype. Using immunofluorescent microscopy, we were able to detect NP+ GCs in the spleens of noninfected mice, but there were no detectible NP+ GCs in mice infected with P. chabaudi. These data suggest that during P. chabaudi infection, there is a shift toward an extrafollicular Ab response that could be responsible for decreased Ab responses to secondary T-dependent Ags.
- Subjects :
- Male
Ovalbumin
T-Lymphocytes
Immunology
Antibodies, Protozoan
Antigens, Protozoan
Spleen
Biology
Plasmodium chabaudi
Interferon-gamma
Mice
Immune system
Antigen
Immunity
parasitic diseases
medicine
Animals
Ficoll
Immunology and Allergy
T independent antigen
Germinal center
Germinal Center
biology.organism_classification
Antigens, T-Independent
Virology
Malaria
Mice, Inbred C57BL
Disease Models, Animal
medicine.anatomical_structure
Immunoglobulin M
Immunoglobulin G
Antibody Formation
Humoral immunity
Subjects
Details
- ISSN :
- 15506606 and 00221767
- Volume :
- 191
- Database :
- OpenAIRE
- Journal :
- The Journal of Immunology
- Accession number :
- edsair.doi.dedup.....a05df92d2029bdc29221b12641abc7fc