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Determination of Bactericidal Activity of Antibacterial Monomer MDPB by a Viability Staining Method
- Source :
- Dental Materials Journal. 27:145-148
- Publication Year :
- 2008
- Publisher :
- Japanese Society for Dental Materials and Devices, 2008.
-
Abstract
- In this study, the bactericidal activity of antibacterial monomer MDPB (12-methacryloyloxydodecylpyridinium bromide) against Streptococcus mutans was tested by a rapid method for monitoring viability. To S. mutans culture containing fluorescence staining solution that distinguishes live from dead cells, MDPB was added at a concentration of 250, 100, 50, or 10 microg/ml. Bacterial cells were observed by fluorescence microscopy and the percentage of dead cells was calculated. After 10, 20, or 30 minutes' contact with MDPB, the live/dead ratio was measured by fluorometry and viable counts (CFU) determined by the conventional plating method. Viability staining revealed that MDPB exhibited significant bactericidal effects at 50 microg/ml or greater (ANOVA, Fisher's PLSD test), and complete killing of the cells at 250 microg/ml of MDPB was demonstrated in conjunction with a plating method. The staining method thus provided a sensitive means to determine loss of viability, and indicated the strong killing effects of MDPB on S. mutans.
- Subjects :
- Time Factors
Chromatography
Dose-Response Relationship, Drug
biology
Chemistry
Colony Count, Microbial
Pyridinium Compounds
biology.organism_classification
Streptococcus mutans
Anti-Bacterial Agents
Microbiology
Staining
Dental Materials
Viability staining
chemistry.chemical_compound
Monomer
Microscopy, Fluorescence
Materials Testing
Ceramics and Composites
Fluorescence microscope
Humans
General Dentistry
Fluorescence staining
Fluorescent Dyes
Subjects
Details
- ISSN :
- 18811361 and 02874547
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Dental Materials Journal
- Accession number :
- edsair.doi.dedup.....a05d4ce30cf07931831e85ada246d49b