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Evaluation of Antiplasmodial activity of extracts and constituents from Ampelozizyphus amazonicus
- Source :
- Pharmacognosy Magazine, Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Publication Year :
- 2015
-
Abstract
- PMID: 26664012 WOS: 000362879000003 BACKGROUND: Ampelozizyphus amazonicus Ducke, a plant that is widely used by the population of the Amazonian region to prevent and treat malaria, was investigated in this work, which describes, for the first time, the antiplasmodial activity of its extracts and associates this activity with its isolated constituents. METHODS: Different extracts with solvents of increasing polarity (hexane, chloroform, ethanol, and water) were obtained of the root bark. This procedure resulted in extracts that were characterized for their constituents. The cytotoxicity and activity of the extracts against Plasmodium berghei (schizontocidal activity, liver stage) and Plasmodium falciparum (3D7 and Dd2 strains, erythrocyte stage) were assessed in vitro. RESULTS: Of the four extracts assayed against P. berghei, the chloroform extract showed the greatest activity, with an inhibitory concentration 50% (IC50) value of 30.1 µg/mL, followed by the aqueous extract (IC50 = 39.9 µg/mL). The chloroform extract exhibited the highest antiplasmodial activity in the erythrocyte stage of P. falciparum, with an IC50 value lower than 15 µg/mL. Fractionation of this more active extract led to the isolation and elucidation of pentacyclic triterpenes, lupeol, betulin and betulinic acid, which showed antiplasmodial activities with IC50 values ranging from 5.6 to 80.30 µM. The most active of these, betulinic acid, was further quantified in the extracts by high-performance liquid chromatography-photodiode array detector analyzes. The higher amount was found in the chloroform extract, which was the most active one against P. falciparum. CONCLUSION: The results obtained in this work may partly explain the popular intake of A. amazonicusas an antimalarial remedy in the Amazon region. publishersversion published
- Subjects :
- Ampelozizyphus
Population
Pharmaceutical Science
antimalarial activity
chemistry.chemical_compound
SDG 3 - Good Health and Well-being
Betulinic acid
triterpenes
Drug Discovery
parasitic diseases
Journal Article
Plasmodium berghei
education
Lupeol
education.field_of_study
Chloroform
Betulin
biology
Traditional medicine
Ampelozyziphus amazonicus
Plasmodium falciparum
biology.organism_classification
chemistry
Biochemistry
rhamnaceae
Original Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Pharmacognosy Magazine, Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Accession number :
- edsair.doi.dedup.....a054fa0d0567dcbfac45912ce770cce0