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Effects of Huangqin Decoction on ulcerative colitis by targeting estrogen receptor alpha and ameliorating endothelial dysfunction based on system pharmacology
- Source :
- Journal of Ethnopharmacology. 271:113886
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Ethnopharmacological relevance Huangqin Decoction (HQD), a traditional Chinese medicinal (TCM) formula chronicled in Shang Han Lun, has been used to treat gastrointestinal diseases for nearly 1800 years. Objective To investigate the effects and underlying mechanisms of HQD on ulcerative colitis (UC). Methods The bioactive compounds in HQD were obtained from the traditional Chinese medicine systems pharmacology database. Then, the HQD and UC-related targets were analyzed by establishing HQD-Compounds-Targets (H–C-T) and protein-protein interaction (PPI) networks. Enrichment analysis was used for further study. The candidate targets for the effects of HQD on UC were validated using a dextran sulfate sodium-induced UC mouse experiment. Results The results showed that 51 key targets were gained by matching 284 HQD-related targets and 837 UC-related targets. Combined with H–C-T and PPI network analyses, the key targets were divided into endothelial growth, inflammation and signal transcription-related targets. Further experimental validation showed that HQD targeted estrogen receptor alpha (ESR1) and endothelial growth factor receptors to relieve endothelial dysfunction, thereby improving intestinal barrier function. The expression of inflammatory cytokines and signal transducers was suppressed by HQD treatment and inflammation was inhibited. Conclusions HQD may acts on UC via the regulation of targets and pathways related to improving the intestinal mucosal barrier and ameliorating endothelial dysfunction. Additionally, ERS1 may be a new target to explore the mechanisms of UC.
- Subjects :
- Male
Nitric Oxide Synthase Type III
Nitric Oxide Synthase Type II
Inflammation
Nitric Oxide Synthase Type I
Traditional Chinese medicine
Pharmacology
Proinflammatory cytokine
03 medical and health sciences
0302 clinical medicine
Growth factor receptor
Drug Discovery
medicine
Animals
Endothelium
Protein Interaction Maps
Endothelial dysfunction
030304 developmental biology
Mice, Inbred BALB C
0303 health sciences
Vascular Endothelial Growth Factor Receptor-1
business.industry
Dextran Sulfate
Estrogen Receptor alpha
STAT2 Transcription Factor
medicine.disease
Vascular Endothelial Growth Factor Receptor-2
Ulcerative colitis
ErbB Receptors
Disease Models, Animal
STAT1 Transcription Factor
Cyclooxygenase 2
030220 oncology & carcinogenesis
Colitis, Ulcerative
medicine.symptom
business
Estrogen receptor alpha
Drugs, Chinese Herbal
Scutellaria baicalensis
Systems pharmacology
Subjects
Details
- ISSN :
- 03788741
- Volume :
- 271
- Database :
- OpenAIRE
- Journal :
- Journal of Ethnopharmacology
- Accession number :
- edsair.doi.dedup.....a039c2a6131a739032877bfac48b770e