Back to Search
Start Over
Peripheral administration of κ-opioid receptor antagonist stimulates gonadotropin-releasing hormone pulse generator activity in ovariectomized, estrogen-treated female goats
- Source :
- SC30201902220018, NARO成果DBa
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Pulsatile gonadotropin-releasing hormone (GnRH) secretion is indispensable for reproduction in mammals. Kisspeptin neurons in the hypothalamic arcuate nucleus (ARC), referred to as KNDy neurons because of the coexpression of neurokinin B and dynorphin A, are considered as components of the GnRH pulse generator that produces rhythmic GnRH secretion. The present study aimed to investigate if peripheral administration of PF-4455242, a κ-opioid receptor (KOR, a dynorphin A receptor) antagonist, facilitates pulsatile luteinizing hormone (LH) secretion and GnRH pulse generator activity in estrogen-treated ovariectomized Shiba goats to determine the possibility of using KOR antagonists to artificially control ovarian activities. PF-4455242 was intravenously infused for 4 h (1 or 10 μmol/kg body weight/4 h) or as a single subcutaneous injection (1 or 10 μmol/kg body weight). In a separate experiment, the same KOR antagonist (10 μmol/kg body weight/4 h) was intravenously infused during the recording of multiple unit activity (MUA) in the ARC that reflects the activity of the GnRH pulse generator to test the effects of KOR antagonist administration on GnRH pulse generator activity. Intravenous infusion and single subcutaneous injection of the KOR antagonist significantly increased the frequency of LH pulses compared with controls. Intravenous infusion of KOR antagonist also significantly increased the frequency of episodic bursts in the MUA. The present study demonstrates that peripherally administered KOR antagonist stimulates pulsatile LH secretion by acting on the GnRH pulse generator, and peripheral administration of PF-4455242 can be used to facilitate pulsatile LH secretion, which in turn facilitates ovarian activities in farm animals.
- Subjects :
- endocrine system
medicine.medical_specialty
medicine.drug_class
Injections, Subcutaneous
Ovariectomy
Gonadotropin-releasing hormone
Gonadotropin-Releasing Hormone
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
Kisspeptin
Food Animals
Opioid receptor
Internal medicine
medicine
Animals
Sulfonamides
030219 obstetrics & reproductive medicine
business.industry
Goats
Receptors, Opioid, kappa
Biphenyl Compounds
0402 animal and dairy science
Antagonist
Dynorphin A
Estrogens
04 agricultural and veterinary sciences
040201 dairy & animal science
Gene Expression Regulation
chemistry
Injections, Intravenous
Ovariectomized rat
Female
Animal Science and Zoology
Neurokinin B
business
Luteinizing hormone
Subjects
Details
- ISSN :
- 07397240
- Volume :
- 68
- Database :
- OpenAIRE
- Journal :
- Domestic Animal Endocrinology
- Accession number :
- edsair.doi.dedup.....a0366f5ee34a31f0ecda6638b32b6269
- Full Text :
- https://doi.org/10.1016/j.domaniend.2018.12.011