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Molecular Bioburden of the Lens Storage Case for Contact Lens–Related Keratitis
- Source :
- Cornea. 37:1542-1550
- Publication Year :
- 2018
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2018.
-
Abstract
- PURPOSE To elucidate the bioburden of a lens care system in patients with contact lens (CL)-related keratitis. METHODS In this prospective case-control study, the CL storage case was used as the target of bioburden assessment. Participants were CL wearers with (n = 26) or without (n = 24) keratitis in southern Taiwan. The case fluid sample was consecutively collected and assessed using a dot hybridization assay (DHA) for bioburden assessment. The DHA was composed of 3 universal bacterial probes for detecting all bacteria, genus-specific probes for Pseudomonas and Acinetobacter, and 1 probe for Acanthamoeba. The receiver operating characteristic curve was used to analyze the differentiating performance of a probe. RESULTS The storage case bioburden was significantly higher in those with CL-related keratitis than in control subjects; patients with CL-related keratitis had stronger standardized signals in all universal bacterial probes. Moreover, in the cases of these patients, the bioburden was significantly heavier in confirmed infectious keratitis than in presumed noninfectious keratitis. Receiver operating characteristic analysis showed that stronger signals in all 3 universal bacterial probes and the Pseudomonas probe might suggest that the wearers have infectious keratitis. Only the storage case of the Acanthamoeba keratitis case showed positive detection by the Acanthamoeba probe. CONCLUSIONS A heavier bioburden in the lens storage case was associated with a higher risk of CL-related keratitis and infectious keratitis. Inappropriate maintenance of the CL will lead to microbial contamination and transfer the pathogen onto the ocular surface causing keratitis accordingly. The DHA assessment for the lens storage case might provide an alternative way to differentiate infectious from noninfectious CL-related keratitis.
- Subjects :
- Adult
DNA, Bacterial
Male
0301 basic medicine
medicine.medical_specialty
Contact Lenses
Taiwan
Infectious Keratitis
Polymerase Chain Reaction
Keratitis
Bioburden
Young Adult
03 medical and health sciences
0302 clinical medicine
Ophthalmology
Product Packaging
medicine
Humans
Eye Infections, Parasitic
Prospective Studies
biology
business.industry
Incidence
DNA, Protozoan
Acinetobacter
medicine.disease
biology.organism_classification
Acanthamoeba
Contact lens
030104 developmental biology
medicine.anatomical_structure
Acanthamoeba Keratitis
Acanthamoeba keratitis
Biofilms
Case-Control Studies
Lens (anatomy)
030221 ophthalmology & optometry
Equipment Contamination
Female
Contact Lens Solutions
business
Subjects
Details
- ISSN :
- 02773740
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Cornea
- Accession number :
- edsair.doi.dedup.....a030b40684d59ddd79dc52c9013a6990