Back to Search
Start Over
Multi-flow channel bioreactor enables real-time monitoring of cellular dynamics in 3D engineered tissue
- Source :
- Communications Biology, Communications Biology, Vol 2, Iss 1, Pp 1-10 (2019)
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- The key to understanding, harnessing, and manipulating natural biological processes for the benefit of tissue engineering lies in providing a controllable dynamic environment for tissue development in vitro while being able to track cell activity in real time. This work presents a multi-channel bioreactor specifically designed to enable on-line imaging of fluorescently labeled cells embedded in replicated 3D engineered constructs subjected to different flow conditions. The images are acquired in 3D using a standard upright confocal microscope and further analyzed and quantified by computer vision. The platform is used to characterize and quantify the pace and directionality of angiogenic processes induced by flow. The presented apparatus bears considerable potential to advance scientific research, from basic research pursuing the effect of flow versus static conditions on 3D scaffolds and cell types, to clinically oriented modeling in drug screening and cytotoxicity assays.<br />Zohar et al. demonstrate a real-time imaging of fluorescently labeled cells embedded in 3D constructs subjected to different flow conditions. This study provides a framework that enables tracking cell activity continuously during tissue development, suggesting its potential utility for drug screens or cytotoxicity assays.
- Subjects :
- Vascular Endothelial Growth Factor A
Computer science
Confocal
Cell Culture Techniques
Neovascularization, Physiologic
Medicine (miscellaneous)
Multi flow
02 engineering and technology
Article
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Bioreactors
Imaging, Three-Dimensional
Tissue engineering
Bioreactor
Humans
Cellular dynamics
lcsh:QH301-705.5
Biological models
030304 developmental biology
Biophysical methods
0303 health sciences
Microscopy, Confocal
Tissue Engineering
Tissue Scaffolds
Endothelial Cells
021001 nanoscience & nanotechnology
Perfusion
Flow conditions
lcsh:Biology (General)
Flow (mathematics)
Angiogenesis
Rheology
0210 nano-technology
General Agricultural and Biological Sciences
Biological system
Biotechnology
Communication channel
Subjects
Details
- ISSN :
- 23993642
- Volume :
- 2
- Database :
- OpenAIRE
- Journal :
- Communications Biology
- Accession number :
- edsair.doi.dedup.....a02a24fcc9e42393947f62d1d65a890c